Enriching miRNA-mRNA relationship with miRNA binding site’s sequence profiling and its surrounding environment
Advisor Information
Dhundy Bastola
Location
Milo Bail Student Center Omaha Room
Presentation Type
Oral Presentation
Start Date
8-3-2013 9:45 AM
End Date
8-3-2013 10:00 AM
Abstract
MicroRNAs are small (approx. 22nt) noncoding RNAs that regulate gene expression by either degrading messenger-RNA (mRNA) that has already been transcribed or by repressing the translation of mRNA. This mechanism of gene regulation by binding of the miRNA to 3-prime-UTR of target mRNAs has been recently discovered and sequence–specific post-transcriptional gene regulation process affects large set of genes involved in number of biological pathways. Mapping of 7nt long miRNA seed sequence to the target gene has been a standard way of predicting miRNA targets. In this study, we have generated a sequence profile-based filter to increase the specificity of human miRNA-mRNA relationship thereby enriching true-positive miRNA target sites in humans based on sequence information. We had integrated the role of binding site’s surrounding environment in term of its flanking sequence to further enrich the true miRNA target sites.
Enriching miRNA-mRNA relationship with miRNA binding site’s sequence profiling and its surrounding environment
Milo Bail Student Center Omaha Room
MicroRNAs are small (approx. 22nt) noncoding RNAs that regulate gene expression by either degrading messenger-RNA (mRNA) that has already been transcribed or by repressing the translation of mRNA. This mechanism of gene regulation by binding of the miRNA to 3-prime-UTR of target mRNAs has been recently discovered and sequence–specific post-transcriptional gene regulation process affects large set of genes involved in number of biological pathways. Mapping of 7nt long miRNA seed sequence to the target gene has been a standard way of predicting miRNA targets. In this study, we have generated a sequence profile-based filter to increase the specificity of human miRNA-mRNA relationship thereby enriching true-positive miRNA target sites in humans based on sequence information. We had integrated the role of binding site’s surrounding environment in term of its flanking sequence to further enrich the true miRNA target sites.