Development of RNA Primers and TaqMAN probes for Toxoplasma BAG1 and Ribosomal protein S23

Advisor Information

Paul Davis

Location

UNO Criss Library, Room 249

Presentation Type

Oral Presentation

Start Date

6-3-2015 3:00 PM

End Date

6-3-2015 3:15 PM

Abstract

The quantitative analysis of bradyzoite and tachyzoite stages of the obligate, intracellular, parasite Toxoplasma gondii is possible with the use of qPCR. With the primers for Bradyzoite Antigen1(BAG1) and Ribosomal Protein S23 (RPS23), the expression of each gene was tested during both phases of the parasite. These allow us to evaluate the intensity of the bradyzoite stage generated using in vitro and in vivo methods.

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Mar 6th, 3:00 PM Mar 6th, 3:15 PM

Development of RNA Primers and TaqMAN probes for Toxoplasma BAG1 and Ribosomal protein S23

UNO Criss Library, Room 249

The quantitative analysis of bradyzoite and tachyzoite stages of the obligate, intracellular, parasite Toxoplasma gondii is possible with the use of qPCR. With the primers for Bradyzoite Antigen1(BAG1) and Ribosomal Protein S23 (RPS23), the expression of each gene was tested during both phases of the parasite. These allow us to evaluate the intensity of the bradyzoite stage generated using in vitro and in vivo methods.