Human skeletal muscle mRNA response to exercise in a hot environment
Advisor Information
Dustin Slivka
Location
UNO Criss Library, Room 232
Presentation Type
Oral Presentation
Start Date
6-3-2015 9:15 AM
End Date
6-3-2015 9:30 AM
Abstract
Mitochondrial adaptation is important for both maintaining optimal health as well as improving athletic The purpose of this investigation was to determine the effects of exercise in a hot environment on mitochondrial biogenesisrelated gene expression in human skeletal muscle. Recreationally-active males (n = 9, 25 ± 4 y, 179 ± 4 cm, 76.2 ± 8.0 kg, VO2 peak 4.39 ± 0.82 L · min-1, 13.4 ± 3.3% body fat) completed two experimental trials in which they cycled for 1 h at 60% of Wmax in an environmental temperature of either 20º C (N) or 33º C (H). Muscle biopsies were obtained from the vastus lateralis pre- and 3 h post-exercise for determination of gene expression. Relative oxygen consumption was higher during exercise in H (69.8 ± 4.7%) than N (64.5 ± 5.7%; p = 0.004), as was HR (H: 163 ± 9 bpm, N: 151 ± 9 bpm; p < 0.001). There was a tendency for expression of PGC-1α to be lower following H than N (p = 0.083). Expression of ERRα (p = 0.009), GABPA (p = 0.010), MEF2A (p = 0.080), NRF-1 (p = 0.004), and VEGF (p = 0.004) was blunted following exercise in H as compared to N. Expression of PPARG, SIRT-1, and TFAM was unaffected by temperature or exercise (p = 0.305, p = 0.103, p = 0.410, respectively). These data demonstrate that exercise in a hot environment blunts expression of several genes related to mitochondrial biogenesis.
Human skeletal muscle mRNA response to exercise in a hot environment
UNO Criss Library, Room 232
Mitochondrial adaptation is important for both maintaining optimal health as well as improving athletic The purpose of this investigation was to determine the effects of exercise in a hot environment on mitochondrial biogenesisrelated gene expression in human skeletal muscle. Recreationally-active males (n = 9, 25 ± 4 y, 179 ± 4 cm, 76.2 ± 8.0 kg, VO2 peak 4.39 ± 0.82 L · min-1, 13.4 ± 3.3% body fat) completed two experimental trials in which they cycled for 1 h at 60% of Wmax in an environmental temperature of either 20º C (N) or 33º C (H). Muscle biopsies were obtained from the vastus lateralis pre- and 3 h post-exercise for determination of gene expression. Relative oxygen consumption was higher during exercise in H (69.8 ± 4.7%) than N (64.5 ± 5.7%; p = 0.004), as was HR (H: 163 ± 9 bpm, N: 151 ± 9 bpm; p < 0.001). There was a tendency for expression of PGC-1α to be lower following H than N (p = 0.083). Expression of ERRα (p = 0.009), GABPA (p = 0.010), MEF2A (p = 0.080), NRF-1 (p = 0.004), and VEGF (p = 0.004) was blunted following exercise in H as compared to N. Expression of PPARG, SIRT-1, and TFAM was unaffected by temperature or exercise (p = 0.305, p = 0.103, p = 0.410, respectively). These data demonstrate that exercise in a hot environment blunts expression of several genes related to mitochondrial biogenesis.