Attempted transcriptome assembly of Homalodisca vitripennis

Advisor Information

Paul Davis

Location

UNO Criss Library, Room 112

Presentation Type

Oral Presentation

Start Date

6-3-2015 9:30 AM

End Date

6-3-2015 9:45 AM

Abstract

Homalodisca vitripennis is a large herbivorous insect that feeds on a wide variety of plant species in its nymph and adult forms. Targets of H. vitripennis include plants which produce citrus fruits, grapes, almonds and stone fruits. The purpose of this project is to provide an analysis of RNA sequence data provided by the United States Department of Agriculture (USDA) specifically to the Davis Lab at UNO. Due to RNA quality issues, efforts were placed to aggressively filter and assemble the sequencer data. Differences in the quality of results obtained applying these established protocols to this dataset and to a previous data set in this lab are presented here.

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COinS
 
Mar 6th, 9:30 AM Mar 6th, 9:45 AM

Attempted transcriptome assembly of Homalodisca vitripennis

UNO Criss Library, Room 112

Homalodisca vitripennis is a large herbivorous insect that feeds on a wide variety of plant species in its nymph and adult forms. Targets of H. vitripennis include plants which produce citrus fruits, grapes, almonds and stone fruits. The purpose of this project is to provide an analysis of RNA sequence data provided by the United States Department of Agriculture (USDA) specifically to the Davis Lab at UNO. Due to RNA quality issues, efforts were placed to aggressively filter and assemble the sequencer data. Differences in the quality of results obtained applying these established protocols to this dataset and to a previous data set in this lab are presented here.