De novo Assembly and Analysis of the Chilean Pencil Catfish Trichomycterus areolatus Transcriptome
Advisor Information
Paul Davis
Location
UNO Criss Library, Room 232
Presentation Type
Oral Presentation
Start Date
4-3-2016 10:45 AM
End Date
4-3-2016 11:00 AM
Abstract
The pencil catfish Trichomycterus areolatus is native to Chile and a potential sentinel organism to detect agricultural runoff. T. areolatus is currently suffering population decline for unknown causes. This catfish is found in freshwater streams and is attracted to lower levels of water bodies. We detail the assembly and validation of the T. areolatus transcriptome, a molecular resource for the study of this organism and its environment. RNA-Seq reads were obtained by next-generation sequencing with an Illumina platform and processed using PRINSEQ. The assembly was performed using TRINITY assembler. Transcriptome annotation was performed with KOG, KEGG, and GO analysis. Additionally, differential expression analysis was included to detail tissue-specific expression patterns. A select list of endocrine related transcripts were included.
De novo Assembly and Analysis of the Chilean Pencil Catfish Trichomycterus areolatus Transcriptome
UNO Criss Library, Room 232
The pencil catfish Trichomycterus areolatus is native to Chile and a potential sentinel organism to detect agricultural runoff. T. areolatus is currently suffering population decline for unknown causes. This catfish is found in freshwater streams and is attracted to lower levels of water bodies. We detail the assembly and validation of the T. areolatus transcriptome, a molecular resource for the study of this organism and its environment. RNA-Seq reads were obtained by next-generation sequencing with an Illumina platform and processed using PRINSEQ. The assembly was performed using TRINITY assembler. Transcriptome annotation was performed with KOG, KEGG, and GO analysis. Additionally, differential expression analysis was included to detail tissue-specific expression patterns. A select list of endocrine related transcripts were included.