Human Skeletal Muscle Proteolytic Gene Expression after Exposure to Different Environmental Temperatures
Advisor Information
Dustin Slivka
Location
Dr. C.C. and Mabel L. Criss Library
Presentation Type
Poster
Start Date
4-3-2016 2:30 PM
End Date
4-3-2016 4:00 PM
Abstract
Temperature combined with exercise haven been shown to effect several genes related to skeletal muscle proteolysis in humans. However, the effects of temperature exposure independent of exercise on proteolytic gene expression have not been determined. Purpose: To determine the effects of acute hot, cold, and room temperature exposure on skeletal muscle gene expression related to proteolysis in humans. Methods: Twelve recreationally trained male subjects had skeletal muscle biopsies taken from the vastus lateralis before and after 3 hours of sitting in an environmentally controlled chamber in either hot (H), cold (C), or room temperature (RT), conditions (33 ºC, 7 ºC, 20 ºC, respectively). Results: There was no effect of temperature on gene expression of atrogin-1, MuRF1, or FOXO3 (p = 0.543, p = 0.693, p = 0.102, respectively). However, FOXO3 decreased with time regardless of temperature (p = 0.004). Core temperature was significantly higher in H and C compared to RT (37.2 ± 0.1ºC, p = 0.001; 37.1 ± 0.1ºC, p = 0.013; 36.9 ± 0.1ºC, respectively). Whole body oxygen consumption was significantly higher in H and C compared to RT (0.38 ± 0.01 L∙min-1, p < 0.001; 0.52 ± 0.03 L∙min-1, p = 0.001; 0.35 ± 0.01 L∙min-1, respectively). Conclusions: These results indicate that without an exercise stimulus, acute exposure to hot and cold is not sufficient to alter proteolytic gene expression. When considered with our previous research it appears that exercise and temperature act synergistically to alter the transcriptional response.
Human Skeletal Muscle Proteolytic Gene Expression after Exposure to Different Environmental Temperatures
Dr. C.C. and Mabel L. Criss Library
Temperature combined with exercise haven been shown to effect several genes related to skeletal muscle proteolysis in humans. However, the effects of temperature exposure independent of exercise on proteolytic gene expression have not been determined. Purpose: To determine the effects of acute hot, cold, and room temperature exposure on skeletal muscle gene expression related to proteolysis in humans. Methods: Twelve recreationally trained male subjects had skeletal muscle biopsies taken from the vastus lateralis before and after 3 hours of sitting in an environmentally controlled chamber in either hot (H), cold (C), or room temperature (RT), conditions (33 ºC, 7 ºC, 20 ºC, respectively). Results: There was no effect of temperature on gene expression of atrogin-1, MuRF1, or FOXO3 (p = 0.543, p = 0.693, p = 0.102, respectively). However, FOXO3 decreased with time regardless of temperature (p = 0.004). Core temperature was significantly higher in H and C compared to RT (37.2 ± 0.1ºC, p = 0.001; 37.1 ± 0.1ºC, p = 0.013; 36.9 ± 0.1ºC, respectively). Whole body oxygen consumption was significantly higher in H and C compared to RT (0.38 ± 0.01 L∙min-1, p < 0.001; 0.52 ± 0.03 L∙min-1, p = 0.001; 0.35 ± 0.01 L∙min-1, respectively). Conclusions: These results indicate that without an exercise stimulus, acute exposure to hot and cold is not sufficient to alter proteolytic gene expression. When considered with our previous research it appears that exercise and temperature act synergistically to alter the transcriptional response.