Human Skeletal Muscle Myogenic Gene Expression After Exposure to Different Environmental Temperatures

Advisor Information

Dustin Slivka

Location

Dr. C.C. and Mabel L. Criss Library

Presentation Type

Poster

Start Date

4-3-2016 9:00 AM

End Date

4-3-2016 10:30 AM

Abstract

When combined with exercise, temperature has been demonstrated to be a potent stimulus for the expression of genes related to myogenesis (muscle growth). The impact of environmental temperature, independent of exercise, on myogenesis has not been investigated. Purpose: To determine the acute effects of hot, cold, and room temperature exposure on human skeletal muscle gene expression related to myogenesis. Methods: Twelve recreationally trained male subjects each participated in 3 trials: hot (H), cold (C), and room temperature (RT) conditions. Muscle biopsies were taken from the vastus lateralis before and after 3 hours of sitting in an environmentally controlled chamber at 33 ºC (H), 7 ºC (C), or 20 ºC (RT). Results: There was no effect of temperature on gene expression of MSTN (p = 0.987), MYOG (p = 0.444), MYF5 (p = 0.343), and MYF6 (p = 0.458). However, MSTN and MYF5 decreased (p < 0.001, p = 0.003, respectively) while MYF6 increased (p = 0.026) with time regardless of temperature. Core temperature was significantly higher in H (37.2 ± 0.1 ºC, p = 0.001) and C (37.1 ± 0.1 ºC, p = 0.013) compared to RT (36.9 ± 0.1 ºC). Whole body oxygen consumption was significantly higher in H (0.38 ± 0.01 L∙min-1, p < 0.001) and C (0.52 ± 0.03 L∙min-1, p = 0.001) compared to RT (0.35 ± 0.01 L∙min-1). Conclusions: When considered in conjunction with previous research, exercise appears to be a necessary component for temperature induced gene expression alterations related to muscle growth in humans.

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Mar 4th, 9:00 AM Mar 4th, 10:30 AM

Human Skeletal Muscle Myogenic Gene Expression After Exposure to Different Environmental Temperatures

Dr. C.C. and Mabel L. Criss Library

When combined with exercise, temperature has been demonstrated to be a potent stimulus for the expression of genes related to myogenesis (muscle growth). The impact of environmental temperature, independent of exercise, on myogenesis has not been investigated. Purpose: To determine the acute effects of hot, cold, and room temperature exposure on human skeletal muscle gene expression related to myogenesis. Methods: Twelve recreationally trained male subjects each participated in 3 trials: hot (H), cold (C), and room temperature (RT) conditions. Muscle biopsies were taken from the vastus lateralis before and after 3 hours of sitting in an environmentally controlled chamber at 33 ºC (H), 7 ºC (C), or 20 ºC (RT). Results: There was no effect of temperature on gene expression of MSTN (p = 0.987), MYOG (p = 0.444), MYF5 (p = 0.343), and MYF6 (p = 0.458). However, MSTN and MYF5 decreased (p < 0.001, p = 0.003, respectively) while MYF6 increased (p = 0.026) with time regardless of temperature. Core temperature was significantly higher in H (37.2 ± 0.1 ºC, p = 0.001) and C (37.1 ± 0.1 ºC, p = 0.013) compared to RT (36.9 ± 0.1 ºC). Whole body oxygen consumption was significantly higher in H (0.38 ± 0.01 L∙min-1, p < 0.001) and C (0.52 ± 0.03 L∙min-1, p = 0.001) compared to RT (0.35 ± 0.01 L∙min-1). Conclusions: When considered in conjunction with previous research, exercise appears to be a necessary component for temperature induced gene expression alterations related to muscle growth in humans.