Presenter Type
UNO Undergraduate Student
Major/Field of Study
Biology
Other
Molecular and Biomedical Biology
Advisor Information
Advised by Jonathan B. Clayton, Ph.D.
Location
CEC RM #201/205/209
Presentation Type
Poster
Poster Size
48x36
Start Date
22-3-2024 1:00 PM
End Date
22-3-2024 2:15 PM
Abstract
Materials and methods developed for the recording and analysis of behavior in the common marmoset (Callithrix jacchus).
Christian J. Wintlea, Jordan B. Hernandeza,b,c, Dobromir Dotovd, and Jonathan B. Claytona,b,e,f,g
aDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA
bNebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA
cDepartment of Genetics, Cell Biology, and Anatomy, University of Nebraska Medical Center, NE, USA
dDepartment of Biomechanics, University of Nebraska at Omaha, Omaha, NE, USA
eDepartment of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA
fDepartment of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA
gPrimate Microbiome Project, University of Nebraska-Lincoln, Lincoln, NE, USA
Behavioral data is essential to the recognition of behavioral cues that indicate changes in mental wellness and overall health of an individual. In the common marmoset (Callithrix jacchus), behavioral analysis requires the continuous observation and scoring of behavior using an ethogram. Ethograms for the common marmoset contain actions and behaviors used to objectively judge the individual's action in a systematic way. Cameras are a useful method for the collection and then post recording analyses of data. In the desire for collection of common marmoset behavioral data at the Callitrichid Research Center (CRC) located at the University of Nebraska at Omaha, many factors were taken into consideration to determine the most effective and practical camera. Cameras were chosen based on battery life, storage requirements, resolution and quality of video, file export type, overall simplicity and space requirements permitted by the housing of the marmosets in the CRC. The camera chosen to use for the upcoming research study is the Insta360 (ONE RS 1-inch 360 edition). The Insta360 camera is designed for up to 2 hours of recording at 6K resolution in a 360 degree field of view along with manipulation of video sharpness, clarity, types of camera views pre and post recording as well as a strong, reliable camera durability. Initiation of the next aim in an ongoing experiment is set to start in the next month in which several of the Insta360 cameras will be used to record common marmoset behavior in experimental and control groups alike. This data will then be analyzed using a developed ethogram for recognition of behavioral changes due to antibiotic administration and social isolation. Future uses and significance of this data include the possibility to develop a new ethogram for the common marmoset and the reanalysis of video data for new studies.
Included in
Animal Experimentation and Research Commons, Animal Studies Commons, Behavioral Disciplines and Activities Commons, Behavior and Behavior Mechanisms Commons, Behavior and Ethology Commons, Microbiology Commons, Other Animal Sciences Commons
Materials and Methods Developed for the Recording and Analysis of Behavior in the Common Marmoset (Callithrix jacchus).
CEC RM #201/205/209
Materials and methods developed for the recording and analysis of behavior in the common marmoset (Callithrix jacchus).
Christian J. Wintlea, Jordan B. Hernandeza,b,c, Dobromir Dotovd, and Jonathan B. Claytona,b,e,f,g
aDepartment of Biology, University of Nebraska at Omaha, Omaha, NE, USA
bNebraska Food for Health Center, University of Nebraska-Lincoln, Lincoln, NE, USA
cDepartment of Genetics, Cell Biology, and Anatomy, University of Nebraska Medical Center, NE, USA
dDepartment of Biomechanics, University of Nebraska at Omaha, Omaha, NE, USA
eDepartment of Food Science and Technology, University of Nebraska-Lincoln, Lincoln, NE, USA
fDepartment of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA
gPrimate Microbiome Project, University of Nebraska-Lincoln, Lincoln, NE, USA
Behavioral data is essential to the recognition of behavioral cues that indicate changes in mental wellness and overall health of an individual. In the common marmoset (Callithrix jacchus), behavioral analysis requires the continuous observation and scoring of behavior using an ethogram. Ethograms for the common marmoset contain actions and behaviors used to objectively judge the individual's action in a systematic way. Cameras are a useful method for the collection and then post recording analyses of data. In the desire for collection of common marmoset behavioral data at the Callitrichid Research Center (CRC) located at the University of Nebraska at Omaha, many factors were taken into consideration to determine the most effective and practical camera. Cameras were chosen based on battery life, storage requirements, resolution and quality of video, file export type, overall simplicity and space requirements permitted by the housing of the marmosets in the CRC. The camera chosen to use for the upcoming research study is the Insta360 (ONE RS 1-inch 360 edition). The Insta360 camera is designed for up to 2 hours of recording at 6K resolution in a 360 degree field of view along with manipulation of video sharpness, clarity, types of camera views pre and post recording as well as a strong, reliable camera durability. Initiation of the next aim in an ongoing experiment is set to start in the next month in which several of the Insta360 cameras will be used to record common marmoset behavior in experimental and control groups alike. This data will then be analyzed using a developed ethogram for recognition of behavioral changes due to antibiotic administration and social isolation. Future uses and significance of this data include the possibility to develop a new ethogram for the common marmoset and the reanalysis of video data for new studies.