Date of Award

12-2024

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Dr. James A. Wilson

Abstract

In 2018 a novel population of southern flying squirrels (Glaucomys volans) was discovered in Lincoln, Nebraska, 80 km outside of their known range. Southern flying squirrels are designated as a “Tier-1” species of concern within Nebraska and the origin of this new population is of interest to state biologists. This study will provide insight into the probable origin of this new population by comparing genetic samples from known populations of southern flying squirrels. Museum specimens were collected from two well-established populations in Florida and Texas, as well as samples from individuals native to Nebraska (Indian Cave State Park) to compare the genetic composition of the Lincoln population. Using double digest restriction enzyme associated DNA sequencing and bioinformatics protocols, 6,841 autosomal loci were isolated and analyzed. Results showed that the Lincoln population had genetic characteristics unique to single-event colonized populations that were not present in either the Florida or Texas populations. In addition, the native population at Indian Cave State Park (Nebraska) has intermediate placement with the Lincoln population and Florida and Texas populations making it difficult to ascertain the origin of the Lincoln population. These findings support the hypothesis that the Lincoln, NE population of southern flying squirrels represents a single colonization event, but more data is necessary to determine the specific origin.

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