Presentation Title

Cloning Fluorogen Activating Protein J10 VL for Protein Localization Studies in Candida Albicans

Advisor Information

Jill Blankenship

Location

Dr. C.C. and Mabel L. Criss Library

Presentation Type

Poster

Start Date

7-3-2014 9:00 AM

End Date

7-3-2014 12:00 PM

Abstract

Fluorogen activating proteins (FAPs) are a new class of protein reporters that can generate fluorescence from otherwise non-fluorescent molecules. We are investigating the use of the FAP J10 VL for observation of septin proteins co-localization in the fungal pathogen Candida albicans. Because the universal CTG codon for leucine codes for serine in C. albicans, the FAP molecule of interest, in which 5 CTG codons have been identified, is affected. The purpose of this project is to make a J10 VL FAP tag that can be used in C.albicans. Using site directed mutagenesis and homologous recombination, CTG sites identified will be changed to TTG, a redundant codon for leucine. Gene sequencing will help determine successful mutagenesis and subsequent staining and imaging will help determine the utility of the FAP J10 VL– OTB bipartite fluoromolecule as a fluorescent tool for protein localization.

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COinS
 
Mar 7th, 9:00 AM Mar 7th, 12:00 PM

Cloning Fluorogen Activating Protein J10 VL for Protein Localization Studies in Candida Albicans

Dr. C.C. and Mabel L. Criss Library

Fluorogen activating proteins (FAPs) are a new class of protein reporters that can generate fluorescence from otherwise non-fluorescent molecules. We are investigating the use of the FAP J10 VL for observation of septin proteins co-localization in the fungal pathogen Candida albicans. Because the universal CTG codon for leucine codes for serine in C. albicans, the FAP molecule of interest, in which 5 CTG codons have been identified, is affected. The purpose of this project is to make a J10 VL FAP tag that can be used in C.albicans. Using site directed mutagenesis and homologous recombination, CTG sites identified will be changed to TTG, a redundant codon for leucine. Gene sequencing will help determine successful mutagenesis and subsequent staining and imaging will help determine the utility of the FAP J10 VL– OTB bipartite fluoromolecule as a fluorescent tool for protein localization.