Presentation Title

Human skeletal muscle mRNA response to exercise in a hot environment

Advisor Information

Dustin Slivka

Location

UNO Criss Library, Room 232

Presentation Type

Oral Presentation

Start Date

6-3-2015 9:15 AM

End Date

6-3-2015 9:30 AM

Abstract

Mitochondrial adaptation is important for both maintaining optimal health as well as improving athletic The purpose of this investigation was to determine the effects of exercise in a hot environment on mitochondrial biogenesisrelated gene expression in human skeletal muscle. Recreationally-active males (n = 9, 25 ± 4 y, 179 ± 4 cm, 76.2 ± 8.0 kg, VO2 peak 4.39 ± 0.82 L · min-1, 13.4 ± 3.3% body fat) completed two experimental trials in which they cycled for 1 h at 60% of Wmax in an environmental temperature of either 20º C (N) or 33º C (H). Muscle biopsies were obtained from the vastus lateralis pre- and 3 h post-exercise for determination of gene expression. Relative oxygen consumption was higher during exercise in H (69.8 ± 4.7%) than N (64.5 ± 5.7%; p = 0.004), as was HR (H: 163 ± 9 bpm, N: 151 ± 9 bpm; p < 0.001). There was a tendency for expression of PGC-1α to be lower following H than N (p = 0.083). Expression of ERRα (p = 0.009), GABPA (p = 0.010), MEF2A (p = 0.080), NRF-1 (p = 0.004), and VEGF (p = 0.004) was blunted following exercise in H as compared to N. Expression of PPARG, SIRT-1, and TFAM was unaffected by temperature or exercise (p = 0.305, p = 0.103, p = 0.410, respectively). These data demonstrate that exercise in a hot environment blunts expression of several genes related to mitochondrial biogenesis.

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Mar 6th, 9:15 AM Mar 6th, 9:30 AM

Human skeletal muscle mRNA response to exercise in a hot environment

UNO Criss Library, Room 232

Mitochondrial adaptation is important for both maintaining optimal health as well as improving athletic The purpose of this investigation was to determine the effects of exercise in a hot environment on mitochondrial biogenesisrelated gene expression in human skeletal muscle. Recreationally-active males (n = 9, 25 ± 4 y, 179 ± 4 cm, 76.2 ± 8.0 kg, VO2 peak 4.39 ± 0.82 L · min-1, 13.4 ± 3.3% body fat) completed two experimental trials in which they cycled for 1 h at 60% of Wmax in an environmental temperature of either 20º C (N) or 33º C (H). Muscle biopsies were obtained from the vastus lateralis pre- and 3 h post-exercise for determination of gene expression. Relative oxygen consumption was higher during exercise in H (69.8 ± 4.7%) than N (64.5 ± 5.7%; p = 0.004), as was HR (H: 163 ± 9 bpm, N: 151 ± 9 bpm; p < 0.001). There was a tendency for expression of PGC-1α to be lower following H than N (p = 0.083). Expression of ERRα (p = 0.009), GABPA (p = 0.010), MEF2A (p = 0.080), NRF-1 (p = 0.004), and VEGF (p = 0.004) was blunted following exercise in H as compared to N. Expression of PPARG, SIRT-1, and TFAM was unaffected by temperature or exercise (p = 0.305, p = 0.103, p = 0.410, respectively). These data demonstrate that exercise in a hot environment blunts expression of several genes related to mitochondrial biogenesis.