Presentation Title

Irisin and FNDC5 Responses to Exercise in Different Environmental Conditions

Advisor Information

Dustin Slivka

Location

Dr. C.C. and Mabel L. Criss Library

Presentation Type

Poster

Start Date

4-3-2016 9:00 AM

End Date

4-3-2016 10:30 AM

Abstract

Fibronectin type III domain containing 5 (FNDC5) is a skeletal muscle membrane bound precursor to Irisin. Irisin, a myokine, is involved in stimulating adipose tissue to become more metabolically active in order to produce heat. Purpose: To determine the effects of exercise in a hot (33 °C), cold (7 °C), and room temperature (20 °C) environment on the plasma concentrations of irisin and the skeletal muscle gene expression of FNDC5. Methods: Twelve recreationally trained males completed a 1 h cycling bout at 60% of Wmax, in a hot (H), cold (C), and room temperature (RT) environment followed by three hours of recovery at room temperature. Blood samples were taken from the antecubital vein and muscle biopsies were taken from the vastus lateralis pre-, post-, and 3 h post-exercise. Results: Plasma concentrations of irisin did not change from pre to post-exercise, but did decrease from post-exercise to 3 h post-exercise regardless of temperature (9.6 ± 0.2 pg/mL, 8.9 ± 0.5 pg/mL; p = 0.047, respectively). However, when plasma volume shifts were considered, no difference was found in irisin (p = 0.086). FNDC5 mRNA significantly increased from pre- to post-exercise and no change to 3 h post-exercise (p = 0.025; p = 0.061, respectively). Conclusion: The concentration, but not total amount of irisin decreased with exercise with no impact of temperature. Furthermore, skeletal muscle FNDC5 mRNA is increased with exercise. These data collectively suggest that mechanisms other than FNDC5 transcription regulate irisin release during the early response to acute exercise in humans.

Comments

Winner of Honorable Mention Graduate Poster Presentation

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COinS
 
Mar 4th, 9:00 AM Mar 4th, 10:30 AM

Irisin and FNDC5 Responses to Exercise in Different Environmental Conditions

Dr. C.C. and Mabel L. Criss Library

Fibronectin type III domain containing 5 (FNDC5) is a skeletal muscle membrane bound precursor to Irisin. Irisin, a myokine, is involved in stimulating adipose tissue to become more metabolically active in order to produce heat. Purpose: To determine the effects of exercise in a hot (33 °C), cold (7 °C), and room temperature (20 °C) environment on the plasma concentrations of irisin and the skeletal muscle gene expression of FNDC5. Methods: Twelve recreationally trained males completed a 1 h cycling bout at 60% of Wmax, in a hot (H), cold (C), and room temperature (RT) environment followed by three hours of recovery at room temperature. Blood samples were taken from the antecubital vein and muscle biopsies were taken from the vastus lateralis pre-, post-, and 3 h post-exercise. Results: Plasma concentrations of irisin did not change from pre to post-exercise, but did decrease from post-exercise to 3 h post-exercise regardless of temperature (9.6 ± 0.2 pg/mL, 8.9 ± 0.5 pg/mL; p = 0.047, respectively). However, when plasma volume shifts were considered, no difference was found in irisin (p = 0.086). FNDC5 mRNA significantly increased from pre- to post-exercise and no change to 3 h post-exercise (p = 0.025; p = 0.061, respectively). Conclusion: The concentration, but not total amount of irisin decreased with exercise with no impact of temperature. Furthermore, skeletal muscle FNDC5 mRNA is increased with exercise. These data collectively suggest that mechanisms other than FNDC5 transcription regulate irisin release during the early response to acute exercise in humans.