Presentation Title

Human Skeletal Muscle Mitochondrial Related Gene Expression after Exposure to Different Environmental Temperatures

Advisor Information

Dustin Slivka

Location

Dr. C.C. and Mabel L. Criss Library

Presentation Type

Poster

Start Date

4-3-2016 9:00 AM

End Date

4-3-2016 10:30 AM

Abstract

Recent research has reported an effect of environmental temperature on the exercise stimulated response of several genes related to skeletal muscle mitochondrial biogenesis in humans. However, the impact of environmental temperature, independent of exercise, has not been addressed. Purpose: To determine the effects of acute hot and cold exposure on skeletal muscle gene expression related to mitochondrial biogenesis in humans. Methods: Eleven recreationally trained male subjects had skeletal muscle biopsies taken from the vastus lateralis after 3 hours of seated quiet rest in an environmentally controlled chamber in either cold (C), room temperature (RT), or hot (H) conditions (7ºC, 20ºC, 33ºC, respectively). Results: Core temperature was higher in H and C compared to RT (37.2 ± 0.1ºC, p = 0.001; 37.1 ± 0.1ºC, p = 0.013; 36.9 ± 0.1ºC, respectively). Whole body oxygen consumption was higher in H and C compared to RT (0.38 ± 0.01 L∙min-1, p < 0.001; 0.52 ± 0.03 L∙min-1, p = 0.001; 0.35 ± 0.01 L∙min-1, respectively). There was no difference in the gene expression of ERRα (p = 0.665), GABPA (p = 0.080), MEF2A (p = 0.630), NRF1 (p = 0.651), PGC1α (p = 0.612), SIRT1 (p = 0.080), TFAM (p = 0.890), or VEGF (p = 0.080) between H, C, and RT. Conclusions: Temperature exposure alone does not elicit significant changes in gene expression related to mitochondrial biogenesis. When considered in conjunction with previous research, exercise appears to be a necessary component to observe gene expression alterations between different environmental temperatures in humans.

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Mar 4th, 9:00 AM Mar 4th, 10:30 AM

Human Skeletal Muscle Mitochondrial Related Gene Expression after Exposure to Different Environmental Temperatures

Dr. C.C. and Mabel L. Criss Library

Recent research has reported an effect of environmental temperature on the exercise stimulated response of several genes related to skeletal muscle mitochondrial biogenesis in humans. However, the impact of environmental temperature, independent of exercise, has not been addressed. Purpose: To determine the effects of acute hot and cold exposure on skeletal muscle gene expression related to mitochondrial biogenesis in humans. Methods: Eleven recreationally trained male subjects had skeletal muscle biopsies taken from the vastus lateralis after 3 hours of seated quiet rest in an environmentally controlled chamber in either cold (C), room temperature (RT), or hot (H) conditions (7ºC, 20ºC, 33ºC, respectively). Results: Core temperature was higher in H and C compared to RT (37.2 ± 0.1ºC, p = 0.001; 37.1 ± 0.1ºC, p = 0.013; 36.9 ± 0.1ºC, respectively). Whole body oxygen consumption was higher in H and C compared to RT (0.38 ± 0.01 L∙min-1, p < 0.001; 0.52 ± 0.03 L∙min-1, p = 0.001; 0.35 ± 0.01 L∙min-1, respectively). There was no difference in the gene expression of ERRα (p = 0.665), GABPA (p = 0.080), MEF2A (p = 0.630), NRF1 (p = 0.651), PGC1α (p = 0.612), SIRT1 (p = 0.080), TFAM (p = 0.890), or VEGF (p = 0.080) between H, C, and RT. Conclusions: Temperature exposure alone does not elicit significant changes in gene expression related to mitochondrial biogenesis. When considered in conjunction with previous research, exercise appears to be a necessary component to observe gene expression alterations between different environmental temperatures in humans.