Presentation Title

Human Skeletal Muscle Myogenic Gene Expression After Exposure to Different Environmental Temperatures

Advisor Information

Dustin Slivka

Location

Dr. C.C. and Mabel L. Criss Library

Presentation Type

Poster

Start Date

4-3-2016 9:00 AM

End Date

4-3-2016 10:30 AM

Abstract

When combined with exercise, temperature has been demonstrated to be a potent stimulus for the expression of genes related to myogenesis (muscle growth). The impact of environmental temperature, independent of exercise, on myogenesis has not been investigated. Purpose: To determine the acute effects of hot, cold, and room temperature exposure on human skeletal muscle gene expression related to myogenesis. Methods: Twelve recreationally trained male subjects each participated in 3 trials: hot (H), cold (C), and room temperature (RT) conditions. Muscle biopsies were taken from the vastus lateralis before and after 3 hours of sitting in an environmentally controlled chamber at 33 ºC (H), 7 ºC (C), or 20 ºC (RT). Results: There was no effect of temperature on gene expression of MSTN (p = 0.987), MYOG (p = 0.444), MYF5 (p = 0.343), and MYF6 (p = 0.458). However, MSTN and MYF5 decreased (p < 0.001, p = 0.003, respectively) while MYF6 increased (p = 0.026) with time regardless of temperature. Core temperature was significantly higher in H (37.2 ± 0.1 ºC, p = 0.001) and C (37.1 ± 0.1 ºC, p = 0.013) compared to RT (36.9 ± 0.1 ºC). Whole body oxygen consumption was significantly higher in H (0.38 ± 0.01 L∙min-1, p < 0.001) and C (0.52 ± 0.03 L∙min-1, p = 0.001) compared to RT (0.35 ± 0.01 L∙min-1). Conclusions: When considered in conjunction with previous research, exercise appears to be a necessary component for temperature induced gene expression alterations related to muscle growth in humans.

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Mar 4th, 9:00 AM Mar 4th, 10:30 AM

Human Skeletal Muscle Myogenic Gene Expression After Exposure to Different Environmental Temperatures

Dr. C.C. and Mabel L. Criss Library

When combined with exercise, temperature has been demonstrated to be a potent stimulus for the expression of genes related to myogenesis (muscle growth). The impact of environmental temperature, independent of exercise, on myogenesis has not been investigated. Purpose: To determine the acute effects of hot, cold, and room temperature exposure on human skeletal muscle gene expression related to myogenesis. Methods: Twelve recreationally trained male subjects each participated in 3 trials: hot (H), cold (C), and room temperature (RT) conditions. Muscle biopsies were taken from the vastus lateralis before and after 3 hours of sitting in an environmentally controlled chamber at 33 ºC (H), 7 ºC (C), or 20 ºC (RT). Results: There was no effect of temperature on gene expression of MSTN (p = 0.987), MYOG (p = 0.444), MYF5 (p = 0.343), and MYF6 (p = 0.458). However, MSTN and MYF5 decreased (p < 0.001, p = 0.003, respectively) while MYF6 increased (p = 0.026) with time regardless of temperature. Core temperature was significantly higher in H (37.2 ± 0.1 ºC, p = 0.001) and C (37.1 ± 0.1 ºC, p = 0.013) compared to RT (36.9 ± 0.1 ºC). Whole body oxygen consumption was significantly higher in H (0.38 ± 0.01 L∙min-1, p < 0.001) and C (0.52 ± 0.03 L∙min-1, p = 0.001) compared to RT (0.35 ± 0.01 L∙min-1). Conclusions: When considered in conjunction with previous research, exercise appears to be a necessary component for temperature induced gene expression alterations related to muscle growth in humans.