Presentation Title

Using gold nanoparticles to improve performance of SERS-based immunoassay

Advisor Information

Alexey Krasnoslobodtsev

Location

UNO Criss Library, Room 231

Presentation Type

Oral Presentation

Start Date

4-3-2016 11:30 AM

End Date

4-3-2016 11:45 AM

Abstract

Surface enhanced Raman scattering (SERS) can be used as an efficient readout strategy in immunoassays. SERS outperforms other conventional readout strategies such as fluorescence (as in ELISA) and radioactivity (as in RIA). It has been recently demonstrated that SERS-based immunoassays can discriminate samples of healthy individuals from samples of pancreatic cancer patients. Further improvements in sensitivity and reproducibility are expected to extend the practical applications of the SERS-based immunoassays. One critical component of the SERS-based assay is golden nanoparticles that carry Raman reporter molecules. The nanoparticles also provide enhancement of the Raman signal due to plasmon excitation upon laser illumination. Here we present several strategies designed to improve performance of the SERS-based immunoassay. We also make use of narrow Raman bands to design multiplexed detection platform. First, different protective strategies for golden nanoparticles are presented and discussed. The primary purpose of these strategies is to prevent heating of the particles due to intense laser light, thus enhancing properties of the gold nanoparticles can be longer sustained. Second, we also provide a recipe for creating a multiplexed detection platform. We varied which reporter molecules were added to the nanoparticles and measured the spectra from each. By combining nanoparticles labeled with reporter molecules having different well distinguishable wavelengths, it may be possible to measure levels of several biomarkers simultaneously in a single run.

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Mar 4th, 11:30 AM Mar 4th, 11:45 AM

Using gold nanoparticles to improve performance of SERS-based immunoassay

UNO Criss Library, Room 231

Surface enhanced Raman scattering (SERS) can be used as an efficient readout strategy in immunoassays. SERS outperforms other conventional readout strategies such as fluorescence (as in ELISA) and radioactivity (as in RIA). It has been recently demonstrated that SERS-based immunoassays can discriminate samples of healthy individuals from samples of pancreatic cancer patients. Further improvements in sensitivity and reproducibility are expected to extend the practical applications of the SERS-based immunoassays. One critical component of the SERS-based assay is golden nanoparticles that carry Raman reporter molecules. The nanoparticles also provide enhancement of the Raman signal due to plasmon excitation upon laser illumination. Here we present several strategies designed to improve performance of the SERS-based immunoassay. We also make use of narrow Raman bands to design multiplexed detection platform. First, different protective strategies for golden nanoparticles are presented and discussed. The primary purpose of these strategies is to prevent heating of the particles due to intense laser light, thus enhancing properties of the gold nanoparticles can be longer sustained. Second, we also provide a recipe for creating a multiplexed detection platform. We varied which reporter molecules were added to the nanoparticles and measured the spectra from each. By combining nanoparticles labeled with reporter molecules having different well distinguishable wavelengths, it may be possible to measure levels of several biomarkers simultaneously in a single run.