Presentation Title

Chemical Probe of a Critical Cytosine Cluster in the RNA Genome of Coxsackievirus

Presenter Information

Yusuf AbdirahmanFollow

Advisor Information

William Tapprich

Location

Dr. C.C. and Mabel L. Criss Library

Presentation Type

Poster

Start Date

2-3-2018 9:00 AM

End Date

2-3-2018 10:15 AM

Abstract

Coxsackievirus B3 (CVB3) a picornavirus, is a causative pathogen for several human diseases including myocarditis and Type I diabetes .Previous studies with Polio Virus have identified a cytosine rich region in the 5’UTR (untranslated) RNA genome of Polio virus at positions 93-100 to be of importance to viral-host protein interactions necessary for viral proliferation. This particular region is highly conserved among enteroviruses such as CVB3 and Polio virus. In order to explore the role of the spacer region in CVB3/28 RNA genome we mutated two cytosine’s at positions 93-94 in the 5’UTR with adenosines. We then used chemical probing using Selective 2’- Hydroxyl Acylation analyzed by Primer Extension (SHAPE) to characterize the mutant CVB3/28 strain. Data from SHAPE reactivity values was used to generate 5’UTR structures.

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Mar 2nd, 9:00 AM Mar 2nd, 10:15 AM

Chemical Probe of a Critical Cytosine Cluster in the RNA Genome of Coxsackievirus

Dr. C.C. and Mabel L. Criss Library

Coxsackievirus B3 (CVB3) a picornavirus, is a causative pathogen for several human diseases including myocarditis and Type I diabetes .Previous studies with Polio Virus have identified a cytosine rich region in the 5’UTR (untranslated) RNA genome of Polio virus at positions 93-100 to be of importance to viral-host protein interactions necessary for viral proliferation. This particular region is highly conserved among enteroviruses such as CVB3 and Polio virus. In order to explore the role of the spacer region in CVB3/28 RNA genome we mutated two cytosine’s at positions 93-94 in the 5’UTR with adenosines. We then used chemical probing using Selective 2’- Hydroxyl Acylation analyzed by Primer Extension (SHAPE) to characterize the mutant CVB3/28 strain. Data from SHAPE reactivity values was used to generate 5’UTR structures.