Day to Day and Leg to Leg Variation in Gene Expression

Presenter Information

Zohal AlizaiFollow

Advisor Information

Dustin Slivka

Location

Dr. CC and Mabel L. Criss Library

Presentation Type

Poster

Start Date

2-3-2018 10:45 AM

End Date

2-3-2018 12:00 PM

Abstract

Skeletal Muscle Biopsies have been extensively used in research to investigate the effects of exercise. Sequential biopsies are usually taken before and after exercise to measure the effects of physical activity on gene expression. However, in most studies it is not certain if the measured results are due to physical activity or as a result of artifacts due to multiple biopsies. Multiple biopsies in the same leg have been proven to cause stress to the muscle. Such stress can lead to inflammation and other response pathways that altering the gene expression in the muscle. While some research has been conducted to determine the effects of repeated biopsies and differences between legs, such as inflammation and gene expression, not much research has been done to determine the differences in gene expression of the same leg or between legs on different days. There is some evidence suggesting that using different legs in repeated biopsy studies can limit artifacts and give valid results. The difference in gene expression between legs and day to day variation within a leg have not yet been investigated. The purpose of this study is to determine the day to day variance in skeletal muscle gene expression in the same leg and between legs to establish an experimental design that eliminates artifacts and reduces the number of biopsies needed for a study.

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Mar 2nd, 10:45 AM Mar 2nd, 12:00 PM

Day to Day and Leg to Leg Variation in Gene Expression

Dr. CC and Mabel L. Criss Library

Skeletal Muscle Biopsies have been extensively used in research to investigate the effects of exercise. Sequential biopsies are usually taken before and after exercise to measure the effects of physical activity on gene expression. However, in most studies it is not certain if the measured results are due to physical activity or as a result of artifacts due to multiple biopsies. Multiple biopsies in the same leg have been proven to cause stress to the muscle. Such stress can lead to inflammation and other response pathways that altering the gene expression in the muscle. While some research has been conducted to determine the effects of repeated biopsies and differences between legs, such as inflammation and gene expression, not much research has been done to determine the differences in gene expression of the same leg or between legs on different days. There is some evidence suggesting that using different legs in repeated biopsy studies can limit artifacts and give valid results. The difference in gene expression between legs and day to day variation within a leg have not yet been investigated. The purpose of this study is to determine the day to day variance in skeletal muscle gene expression in the same leg and between legs to establish an experimental design that eliminates artifacts and reduces the number of biopsies needed for a study.