Presentation Title

Effects of Environmental Temperature and Exercise on Mitochondrial Quantity and Quality

Presenter Information

Halee KellerFollow

Advisor Information

Dustin Slivka

Location

Dr. C.C. and Mabel L. Criss Library

Presentation Type

Poster

Start Date

2-3-2018 12:30 PM

End Date

2-3-2018 1:45 PM

Abstract

EFFECTS OF ENVIRONMENTAL TEMPERATURE AND EXERCISE ON MITOCHONDRIAL QUANTITY AND QUALITY

H. Keller, R. Shute, D. Slivka

University of Nebraska Omaha, Omaha Nebraska

School of Health and Kinesiology, Exercise Physiology Laboratory

Mitochondria play several roles in the regulation processes of the human body, and therefore are found in many different cells. The highest number of mitochondrial DNA can be found within muscle tissue, and is highly susceptible to damage caused by reactive oxygen species (ROS) located nearby in the mitochondrial matrix. MtDNA repair systems are less effective than nuclear DNA systems, which results in dysfunctional mitochondria. Mitochondrial dysfunction has been linked to the aging process as well as many age-related diseases, such as Alzheimer’s disease, various types of cancer, and diabetes. Exercise training increases mitochondrial development within skeletal muscle, and while it may be more difficult for older or diseased populations to exercise, previous work done by our group indicates that environmental temperature may also effect mitochondrial development when it is paired with exercise training. Subjects were performed cycling exercise in hot, cold, or neutral environments (randomized order), and muscle biopsies were taken from the vastus lateralis muscle. The DNA will be isolated and analyzed, using qRT-PCR, which will target the mtMinArc, the mtMajArc, and B2M, a nuclear housekeeping gene. The mtMinArc is a region on mtDNA that is representative of the total number of mtDNA copies present within a sample, and the mtMajArc is a region on mtDNA that is representative of the most common mtDNA deletions. Using these markers, the mtDNA copy number and mtDNA deletion ratio will be compared between temperature environments, in order to identify potential changes with exercise and between environmental conditions.

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Mar 2nd, 12:30 PM Mar 2nd, 1:45 PM

Effects of Environmental Temperature and Exercise on Mitochondrial Quantity and Quality

Dr. C.C. and Mabel L. Criss Library

EFFECTS OF ENVIRONMENTAL TEMPERATURE AND EXERCISE ON MITOCHONDRIAL QUANTITY AND QUALITY

H. Keller, R. Shute, D. Slivka

University of Nebraska Omaha, Omaha Nebraska

School of Health and Kinesiology, Exercise Physiology Laboratory

Mitochondria play several roles in the regulation processes of the human body, and therefore are found in many different cells. The highest number of mitochondrial DNA can be found within muscle tissue, and is highly susceptible to damage caused by reactive oxygen species (ROS) located nearby in the mitochondrial matrix. MtDNA repair systems are less effective than nuclear DNA systems, which results in dysfunctional mitochondria. Mitochondrial dysfunction has been linked to the aging process as well as many age-related diseases, such as Alzheimer’s disease, various types of cancer, and diabetes. Exercise training increases mitochondrial development within skeletal muscle, and while it may be more difficult for older or diseased populations to exercise, previous work done by our group indicates that environmental temperature may also effect mitochondrial development when it is paired with exercise training. Subjects were performed cycling exercise in hot, cold, or neutral environments (randomized order), and muscle biopsies were taken from the vastus lateralis muscle. The DNA will be isolated and analyzed, using qRT-PCR, which will target the mtMinArc, the mtMajArc, and B2M, a nuclear housekeeping gene. The mtMinArc is a region on mtDNA that is representative of the total number of mtDNA copies present within a sample, and the mtMajArc is a region on mtDNA that is representative of the most common mtDNA deletions. Using these markers, the mtDNA copy number and mtDNA deletion ratio will be compared between temperature environments, in order to identify potential changes with exercise and between environmental conditions.