Exercise and Cold-Induced PGC-1a mRNA Isoform-Specific Response
Advisor Information
Dustin Slivka
Location
MBSC 222
Presentation Type
Oral Presentation
Start Date
6-3-2020 9:00 AM
End Date
6-3-2020 10:15 AM
Abstract
Cold exposure with aerobic exercise stimulates gene expression of PGC-1a, the master regulator of mitochondrial biogenesis. PGC-1a can be expressed as multiple isoforms. Among these isoforms is NT-PGC-1a, which produces a truncated form of the PGC-1a protein, as well as isoforms derived from the first exon of the transcript. Relatively little is known about the specificity and response of these isoforms to environmental temperature and aerobic exercise. Purpose: Determine the expression of PGC-1a isoforms following an acute bout of cycling in cold and room temperature conditions. Methods: 9 male participants cycled for 1 hour at 65% Wmax in -2 and 20 °C. A muscle biopsy was taken from the vastus lateralis pre and 3h post exercise. qRT-PCR was used to analyze gene expression of PGC-1a isoforms expression. Results: Gene expression of all isoforms increased due to the exercise intervention 3h post exercise (p < 0.05). At 3h, total PGC-1a was higher in the cold (3.5 ± 0.6 fold increase) compared to room temperature (2.9 ± 0.4 fold increase, p = 0.028). NT-PGC-1a was also higher in cold (4.1 ± 1.1 fold increase) compared to room temperature at 3h (3.4 ± 0.4 fold increase, p = 0.034). Gene expression of exon 1 isoforms was similar in room temperature and cold conditions at 3h post exercise (p > 0.05). Conclusions: Exercise and cold exposure induced alterations in gene expression for total-PGC-1a and its truncated isoform, NT-PGC-1a, but there was no temperature-dependent response in the other PGC-1a isoforms measured.
Exercise and Cold-Induced PGC-1a mRNA Isoform-Specific Response
MBSC 222
Cold exposure with aerobic exercise stimulates gene expression of PGC-1a, the master regulator of mitochondrial biogenesis. PGC-1a can be expressed as multiple isoforms. Among these isoforms is NT-PGC-1a, which produces a truncated form of the PGC-1a protein, as well as isoforms derived from the first exon of the transcript. Relatively little is known about the specificity and response of these isoforms to environmental temperature and aerobic exercise. Purpose: Determine the expression of PGC-1a isoforms following an acute bout of cycling in cold and room temperature conditions. Methods: 9 male participants cycled for 1 hour at 65% Wmax in -2 and 20 °C. A muscle biopsy was taken from the vastus lateralis pre and 3h post exercise. qRT-PCR was used to analyze gene expression of PGC-1a isoforms expression. Results: Gene expression of all isoforms increased due to the exercise intervention 3h post exercise (p < 0.05). At 3h, total PGC-1a was higher in the cold (3.5 ± 0.6 fold increase) compared to room temperature (2.9 ± 0.4 fold increase, p = 0.028). NT-PGC-1a was also higher in cold (4.1 ± 1.1 fold increase) compared to room temperature at 3h (3.4 ± 0.4 fold increase, p = 0.034). Gene expression of exon 1 isoforms was similar in room temperature and cold conditions at 3h post exercise (p > 0.05). Conclusions: Exercise and cold exposure induced alterations in gene expression for total-PGC-1a and its truncated isoform, NT-PGC-1a, but there was no temperature-dependent response in the other PGC-1a isoforms measured.