Presentation Title

Gene Expression Profiles of a Bacteriophage and its Host During Infection

Presenter Information

Elizabeth RussmanFollow

Advisor Information

William Tapprich

Presentation Type

Oral Presentation

Start Date

26-3-2021 12:00 AM

End Date

26-3-2021 12:00 AM

Abstract

RNA sequencing has recently become a major tool to analyze gene expression and is used to study the identity and abundance of RNA transcripts derived from cells. Significant advances in our understanding of infectious processes have come from analyzing RNA sequencing results from virally infected cells. This is true for bacteriophages, also called phages, which are viruses that infect bacteria, and eukaryotic viruses that infect plants and animals. As technologies have developed, RNA sequencing has been used to discover more about what RNA does as a biological molecule under different conditions, including viral infection. This experiment will analyze the total RNA isolated from a bacteriophage infected host bacteria during the infection process using Bioinformatic tools and algorithms. One potential source of Bioinformatic tools is the server called Galaxy, which houses a set of open-source tools that assemble and analyze RNA genomes. Despite the plethora of known phages, many of the genes found in these viruses are still uncharacterized. A novel phage isolated from a water sample taken in New Mexico from a pond located near Vuelta Horcado road that infects the host bacterium Pseudomonas fluorescens will be investigated in this project. The host is a good choice for this experiment because there are few diseases in humans caused by Pseudomonas fluorescens and they are able to live in different host environments (Scales, Dickson, LiPuma, & Huffnagle, 2014).

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Mar 26th, 12:00 AM Mar 26th, 12:00 AM

Gene Expression Profiles of a Bacteriophage and its Host During Infection

RNA sequencing has recently become a major tool to analyze gene expression and is used to study the identity and abundance of RNA transcripts derived from cells. Significant advances in our understanding of infectious processes have come from analyzing RNA sequencing results from virally infected cells. This is true for bacteriophages, also called phages, which are viruses that infect bacteria, and eukaryotic viruses that infect plants and animals. As technologies have developed, RNA sequencing has been used to discover more about what RNA does as a biological molecule under different conditions, including viral infection. This experiment will analyze the total RNA isolated from a bacteriophage infected host bacteria during the infection process using Bioinformatic tools and algorithms. One potential source of Bioinformatic tools is the server called Galaxy, which houses a set of open-source tools that assemble and analyze RNA genomes. Despite the plethora of known phages, many of the genes found in these viruses are still uncharacterized. A novel phage isolated from a water sample taken in New Mexico from a pond located near Vuelta Horcado road that infects the host bacterium Pseudomonas fluorescens will be investigated in this project. The host is a good choice for this experiment because there are few diseases in humans caused by Pseudomonas fluorescens and they are able to live in different host environments (Scales, Dickson, LiPuma, & Huffnagle, 2014).