Presenter Information

Lavanya UppalaFollow

Advisor Information

Dr. William Tapprich, Lydia Phillips

Presentation Type

Oral Presentation

Start Date

26-3-2021 12:00 AM

End Date

26-3-2021 12:00 AM

Abstract

Pseudomonas aeruginosa is a commonly found hospital bacteria, which can cause infections of the blood, pneumonia, and other organs after surgery in humans. It is thought that virus infection of P. aeruginosa enhances bacterial growth by dysregulation of nutritional immunity. Pseudomonas fluorescens is an analog of P. aeruginosa, and causes mild to no symptoms in humans, although it is found in various soils and water samples. Despite their relatively widespread nature, the Pseudomonas family and their bacteriophages still have many proteins and genes that are termed “hypothetical”, and as a result many of their functions are also unknown. Using UNO-SLW2 (G2), a novel podovirus and Pseudomonas phage, to infect P. fluorescens, could elucidate the mechanisms and functions of these genes. These gene expression changes, characterized using RNA sequencing, will provide insight into the phage genes previously unrecognized during infection of P. fluorescens over time and as a stress response.

Comments

I have class from 9am-10am, and 1-3:30pm, as well as a meeting 11am-12pm on March 26th.

Available for download on Thursday, June 08, 3020

COinS
 
Mar 26th, 12:00 AM Mar 26th, 12:00 AM

Characterization of UNO-SLW2 virus infection of Pseudomonas fluorescens using RNA sequencing

Pseudomonas aeruginosa is a commonly found hospital bacteria, which can cause infections of the blood, pneumonia, and other organs after surgery in humans. It is thought that virus infection of P. aeruginosa enhances bacterial growth by dysregulation of nutritional immunity. Pseudomonas fluorescens is an analog of P. aeruginosa, and causes mild to no symptoms in humans, although it is found in various soils and water samples. Despite their relatively widespread nature, the Pseudomonas family and their bacteriophages still have many proteins and genes that are termed “hypothetical”, and as a result many of their functions are also unknown. Using UNO-SLW2 (G2), a novel podovirus and Pseudomonas phage, to infect P. fluorescens, could elucidate the mechanisms and functions of these genes. These gene expression changes, characterized using RNA sequencing, will provide insight into the phage genes previously unrecognized during infection of P. fluorescens over time and as a stress response.