Presentation Title

Influence of Local Muscle Cooling on Mitochondrial-Related Gene Expression

Advisor Information

Dustin Slivka

Presentation Type

Poster

Start Date

26-3-2021 12:00 AM

End Date

26-3-2021 12:00 AM

Abstract

Background: Mitochondrial health is an important factor for preventing disease and optimizing function. Exercise promotes mitochondrial related gene expression in skeletal muscle, specifically upregulated PGC-1α, the master regulator of mitochondrial biogenesis. Additionally, previous studies suggest exercise in cold ambient temperatures can elevate mitochondrial biogenesis gene expression; however, others demonstrate a blunted gene expression when applied to a local area (i.e., vastus lateralis). More importantly, the mitochondrial effects of cooling a muscle at rest is unknown. Purpose: The purpose of this study is to apply localized cooling to the vastus lateralis at rest to examine gene expression related to mitochondrial homeostasis. Methods: A total of 12 apparently healthy individuals (age 19-45) will sit with a cold (0°C) wrap on one leg and a room temperature (21°C) wrap on the other leg (control). After a 4-hour cold treatment, the thermal wraps will be removed, and measurements will be collected for blood flow, skin temperature, intramuscular muscle temperature and a Bergstrom muscle biopsy will be obtained. This biopsy will be used to analyze transcriptional changes of genes associated with mitochondrial growth (biogenesis) and breakdown (mitophagy). Implications: These results will help put in perspective previous research, which evaluated ambient and local cold therapies during exercise, by eliminating the exercise effect. Finally, this study will contribute to a better understanding of how local cold application will affect mitochondria health in skeletal muscle and to the potential development of therapeutic applications.

This document is currently not available here.

COinS
 
Mar 26th, 12:00 AM Mar 26th, 12:00 AM

Influence of Local Muscle Cooling on Mitochondrial-Related Gene Expression

Background: Mitochondrial health is an important factor for preventing disease and optimizing function. Exercise promotes mitochondrial related gene expression in skeletal muscle, specifically upregulated PGC-1α, the master regulator of mitochondrial biogenesis. Additionally, previous studies suggest exercise in cold ambient temperatures can elevate mitochondrial biogenesis gene expression; however, others demonstrate a blunted gene expression when applied to a local area (i.e., vastus lateralis). More importantly, the mitochondrial effects of cooling a muscle at rest is unknown. Purpose: The purpose of this study is to apply localized cooling to the vastus lateralis at rest to examine gene expression related to mitochondrial homeostasis. Methods: A total of 12 apparently healthy individuals (age 19-45) will sit with a cold (0°C) wrap on one leg and a room temperature (21°C) wrap on the other leg (control). After a 4-hour cold treatment, the thermal wraps will be removed, and measurements will be collected for blood flow, skin temperature, intramuscular muscle temperature and a Bergstrom muscle biopsy will be obtained. This biopsy will be used to analyze transcriptional changes of genes associated with mitochondrial growth (biogenesis) and breakdown (mitophagy). Implications: These results will help put in perspective previous research, which evaluated ambient and local cold therapies during exercise, by eliminating the exercise effect. Finally, this study will contribute to a better understanding of how local cold application will affect mitochondria health in skeletal muscle and to the potential development of therapeutic applications.