Presentation Title

The Use of a Resazurin Reduction Assay and a Luciferin ATP Assay to Measure Splenocyte Activity

Presenter Information

Caleb SandallFollow

Advisor Information

Paul H. Davis

Presentation Type

Poster

Start Date

26-3-2021 12:00 AM

End Date

26-3-2021 12:00 AM

Abstract

Schistosomiasis is a condition caused by a parasitic worm infection that predominantly affects third world countries. There has been a novel drug developed that has demonstrated efficacy against worm infection. Preliminary data from our lab suggests that granulocyte surface markers are upregulated alongside these cells’ ability to produce reactive species in murine splenocytes post treatment. Through a resazurin reduction assay and a Luciferin ATP assay, we examined the effects of this novel drug on granulocytes’ ability to produce reactive oxygen species using ATP and NADH.

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Mar 26th, 12:00 AM Mar 26th, 12:00 AM

The Use of a Resazurin Reduction Assay and a Luciferin ATP Assay to Measure Splenocyte Activity

Schistosomiasis is a condition caused by a parasitic worm infection that predominantly affects third world countries. There has been a novel drug developed that has demonstrated efficacy against worm infection. Preliminary data from our lab suggests that granulocyte surface markers are upregulated alongside these cells’ ability to produce reactive species in murine splenocytes post treatment. Through a resazurin reduction assay and a Luciferin ATP assay, we examined the effects of this novel drug on granulocytes’ ability to produce reactive oxygen species using ATP and NADH.