Presentation Title

Utilization of a Density Gradient Centrifugation-Based Human PBMC Isolation Assay

Presenter Information

Alexander ReganFollow

Advisor Information

Dr. Paul W Denton

Location

MBSC Ballroom - Poster #210 - G

Presentation Type

Poster

Start Date

4-3-2022 2:00 PM

End Date

4-3-2022 3:15 PM

Abstract

In the Denton Immunobiology Lab, we research natural killer (NK) cell-mediated combination immunotherapeutic strategies. My primary role in this research is to isolate peripheral blood mononuclear cells (PBMCs) from human blood products. Other investigators in the lab then use the PBMC pools to enrich for their cells of interest (e.g. NK cells and/or innate lymphoid cells). I will present details about the protocol I use and include optimizations that I have performed and incorporated into the workflow. I will present representative cell yields. Details to be shared include: Ficoll density gradient centrifugation, utilization of a red blood cell lysis buffer, optimization of our centrifugation times and speeds, and inclusion of DNAse at key steps to recover the highest yield of viable PBMCs possible.

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COinS
 
Mar 4th, 2:00 PM Mar 4th, 3:15 PM

Utilization of a Density Gradient Centrifugation-Based Human PBMC Isolation Assay

MBSC Ballroom - Poster #210 - G

In the Denton Immunobiology Lab, we research natural killer (NK) cell-mediated combination immunotherapeutic strategies. My primary role in this research is to isolate peripheral blood mononuclear cells (PBMCs) from human blood products. Other investigators in the lab then use the PBMC pools to enrich for their cells of interest (e.g. NK cells and/or innate lymphoid cells). I will present details about the protocol I use and include optimizations that I have performed and incorporated into the workflow. I will present representative cell yields. Details to be shared include: Ficoll density gradient centrifugation, utilization of a red blood cell lysis buffer, optimization of our centrifugation times and speeds, and inclusion of DNAse at key steps to recover the highest yield of viable PBMCs possible.