Presentation Title

Impact of Local Heat Application on Markers of Myogenesis and Proteolysis

Advisor Information

Dustin Slivka

Location

MBSC Ballroom - Poster #606 - G

Presentation Type

Poster

Start Date

4-3-2022 10:45 AM

End Date

4-3-2022 12:00 PM

Abstract

Previous exercise and temperature research indicates that ambient and local application of heat may induce independent effects on muscle gene expression post-exercise. However, it is unclear if an applied local heat stimulus, at rest, may explain changes in markers of myogenesis and proteolysis. PURPOSE: To determine the impact of local heat application on markers of myogenesis and proteolysis in human skeletal muscle at rest. METHODS: Participants (n=12, 26.4±7.2yrs, 176±12cm, 83.6±18.3kg, 19.1±7.7%BF) applied a 4-hour thermal wrap to both legs, experimental (40°C) vs. control (20°C). Muscle samples were collected and processed to extract mRNA and protein. RT-qPCR was completed to measure and myogenic-related and proteolytic-related genes for changes in mRNA (via paired t-test). Proteins were separated and quantified for total protein and phosphorylated protein. RESULTS: Skin temperature (37.4±0.1°C vs. 34.9±0.2°C) and intramuscular temperature (36.3±0.4°C vs. 35.2±0.8°C) increased (both pser2448 phosphorylation was not different (p=0.981), p70Thr389 phosphorylation was not different (p=0.583), and 4E-BP1Thr37/46 phosphorylation was not different (p=0.238). CONCLUSION: Localized heat application on skeletal muscle, at rest, has no influence on gene expression. Thus, additional stimuli (e.g., exercise) are necessary to induce altered markers of myogenesis and proteolysis.

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COinS
 
Mar 4th, 10:45 AM Mar 4th, 12:00 PM

Impact of Local Heat Application on Markers of Myogenesis and Proteolysis

MBSC Ballroom - Poster #606 - G

Previous exercise and temperature research indicates that ambient and local application of heat may induce independent effects on muscle gene expression post-exercise. However, it is unclear if an applied local heat stimulus, at rest, may explain changes in markers of myogenesis and proteolysis. PURPOSE: To determine the impact of local heat application on markers of myogenesis and proteolysis in human skeletal muscle at rest. METHODS: Participants (n=12, 26.4±7.2yrs, 176±12cm, 83.6±18.3kg, 19.1±7.7%BF) applied a 4-hour thermal wrap to both legs, experimental (40°C) vs. control (20°C). Muscle samples were collected and processed to extract mRNA and protein. RT-qPCR was completed to measure and myogenic-related and proteolytic-related genes for changes in mRNA (via paired t-test). Proteins were separated and quantified for total protein and phosphorylated protein. RESULTS: Skin temperature (37.4±0.1°C vs. 34.9±0.2°C) and intramuscular temperature (36.3±0.4°C vs. 35.2±0.8°C) increased (both pser2448 phosphorylation was not different (p=0.981), p70Thr389 phosphorylation was not different (p=0.583), and 4E-BP1Thr37/46 phosphorylation was not different (p=0.238). CONCLUSION: Localized heat application on skeletal muscle, at rest, has no influence on gene expression. Thus, additional stimuli (e.g., exercise) are necessary to induce altered markers of myogenesis and proteolysis.