Presentation Title

Effect of Local Heat Application on Mitochondrial Related Gene Expression

Advisor Information

Dustin Slivka

Location

MBSC Ballroom - Poster #409 - G

Presentation Type

Poster

Start Date

4-3-2022 2:00 PM

End Date

4-3-2022 3:15 PM

Abstract

BACKGROUND: Mitochondrial health is characterized by the constant cycle of growth (biogenesis) and breakdown (mitophagy) within the mitochondria to allow for optimum function. There is a difference in the response of PGC-1α mRNA while exercising in ambient heat compared to local heat. There was a blunting of PGC-1α in ambient heat during exercise whereas there was a non-blunting response of PGC-1α when applying local heat during exercise. However, the effects of local heat application alone, without exercise (i.e., at rest), on skeletal muscle mitochondrial gene expression are unknown. PURPOSE: To determine the impact of local heat at rest on skeletal muscle gene expression related to mitochondrial biogenesis and mitophagy. METHODS: Twelve active subjects sat in a semi-reclined resting position with a heated thermal wrap (HOT) around one thigh and a neutral temperature wrap (CON) around the other (randomized) for 4-hours. Skin temperature, blood flow, intramuscular temperature, and a skeletal muscle biopsy from the vastus lateralis were obtained. RESULTS: Skin temperature was higher during HOT (37.4±0.1°C) than CON (34.9±0.2°C, p0.05). CONCLUSION: These data indicate that heat application alone had no impact on genes related to mitochondrial homeostasis, suggesting other factors are the primary stimulus for alterations in exercise-induced gene expression.

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Mar 4th, 2:00 PM Mar 4th, 3:15 PM

Effect of Local Heat Application on Mitochondrial Related Gene Expression

MBSC Ballroom - Poster #409 - G

BACKGROUND: Mitochondrial health is characterized by the constant cycle of growth (biogenesis) and breakdown (mitophagy) within the mitochondria to allow for optimum function. There is a difference in the response of PGC-1α mRNA while exercising in ambient heat compared to local heat. There was a blunting of PGC-1α in ambient heat during exercise whereas there was a non-blunting response of PGC-1α when applying local heat during exercise. However, the effects of local heat application alone, without exercise (i.e., at rest), on skeletal muscle mitochondrial gene expression are unknown. PURPOSE: To determine the impact of local heat at rest on skeletal muscle gene expression related to mitochondrial biogenesis and mitophagy. METHODS: Twelve active subjects sat in a semi-reclined resting position with a heated thermal wrap (HOT) around one thigh and a neutral temperature wrap (CON) around the other (randomized) for 4-hours. Skin temperature, blood flow, intramuscular temperature, and a skeletal muscle biopsy from the vastus lateralis were obtained. RESULTS: Skin temperature was higher during HOT (37.4±0.1°C) than CON (34.9±0.2°C, p0.05). CONCLUSION: These data indicate that heat application alone had no impact on genes related to mitochondrial homeostasis, suggesting other factors are the primary stimulus for alterations in exercise-induced gene expression.