Date of Award
Master of Arts (MA)
Dr. A. Thomas Weber
Previously a transcript corresponding to a gene in Dictyostelium mucoroides designated A-11 was found in amoebae during vegetative growth and macrocyst formation. The derived amino acid sequence of A-11 was used to search Genbank to find homologous proteins. The highest degree of homology was found with a hemoglobin from the bacterium Vitreoscilla. The Vitreoscilla hemoglobin protein showed greater than 65% similarity and 50% identity with the A-11 amino acid sequence. It was reported that hypoxic conditions could stimulate the expression of the gene corresponding to the Vitreoscilla hemoglobin when cloned in E. coli cells.
Kosinski, Mary E., "The effects of hypoxic conditions on Dictyostelium mucoroides." (1996). Student Work. 3308.