Month/Year of Graduation
Bachelor of Science in Molecular and Biomedical Biology (BTCHBS)
Donald Rowen, PhD
In the context of rising multidrug resistance in biofilm-forming pathogens like Pseudomonas aeruginosa, this study investigates the role of the understudied transcription factor PA5189 in antibiotic resistance and biofilm formation. PA5189 deletion and overexpression mutants were created in a parent P. aeruginosa strain using pEX18Tc-based recombinant suicide vectors, with genotypic verification of putative triparental conjugants achieved through restriction digestion and PCR. The study revealed that PA5189 overexpression significantly increases resistance to commonly used broad spectrum antibiotics such as ciprofloxacin and imipenem. Additionally, differential expression of PA5189 was found to notably affect biofilm formation, with variations contingent on the nutrient composition and salt content of the growth medium. These findings underscore the role of PA5189 in the adaptive mechanisms of P. aeruginosa, contributing to our understanding of the genetic regulation of pathogenicity and offering new insights for targeted antibacterial strategies in the perpetual fight against drug-resistant infections.
Mellick, Seh Na, "Characterizing the role of PA5189 of Pseudomonas aeruginosa in deletion and overexpression mutants" (2024). Theses/Capstones/Creative Projects. 274.