Month/Year of Graduation
5-2025
Degree Name
Bachelor of Science in Molecular and Biomedical Biology (BTCHBS)
Department
Biology
First Advisor
Dr. Kristen Johnson
Abstract
Caffeine is a stimulant well-known for its alertness enhancement and energy functions. Beyond its neurological effects, caffeine also influences cellular qualities, including viability and membrane integrity. This study aimed to investigate the cytotoxic effects of caffeine on two distinct human cell lines: Human Colorectal Adenocarcinoma (HT-29) and Foreskin Fibroblasts (HFF). Cells were exposed to varying concentrations of caffeine to evaluate whether its effects were dose dependent. Cell viability was assessed using the Trypan Blue exclusion and Alamar Blue assays. Results showed that HFF cell death was more evident than the HT-29 Cells, displaying a potential therapeutic for cancer treatment.
Recommended Citation
Srinivasan, Sanjay, "Assessment of Dose-Dependent Cytotoxicity of Caffeine on Human Colorectal Adenocarcinoma and Foreskin Fibroblast Cell Lines" (2025). Theses/Capstones/Creative Projects. 355.
https://digitalcommons.unomaha.edu/university_honors_program/355