Major alteration in coxsackievirus B3 genomic RNA structure distinguishes a virulent strain from an a virulent strain

Authors

Jerome Prusa, University of Nebraska at OmahaFollow
Johanna Missak, University of Nebraska Medical Center
Jeff Kittrell, University of Nebraska Medical Center
John J. Evans, University of Colorado Anshutz Medical Campus
William Tapprich, University of Nebraska at OmahaFollow

Document Type

Article

Publication Date

7-21-2014

Publication Title

Nucleic Acids Research

Volume

42

Issue

15

Abstract

Coxsackievirus B3 (CV-B3) is a cardiovirulent enterovirus that utilizes a 5′ untranslated region (5′UTR) to complete critical viral processes. Here, we directly compared the structure of a 5′UTR from a virulent strain with that of a naturally occurring avirulent strain. Using chemical probing analysis, we identified a structural difference between the two 5′UTRs in the highly substituted stem-loop II region (SLII). For the remainder of the 5′UTR, we observed conserved structure. Comparative sequence analysis of 170 closely related enteroviruses revealed that the SLII region lacks conservation. To investigate independent folding and function, two chimeric CV-B3 strains were created by exchanging nucleotides 104–184 and repeating the 5′UTR structural analysis. Neither the parent SLII nor the remaining domains of the background 5′UTR were structurally altered by the exchange, supporting an independent mechanism of folding and function. We show that the attenuated 5′UTR lacks structure in the SLII cardiovirulence determinant.

Comments

© The Author(s) 2014. https://doi.org/10.1093/nar/gku706

Recommended Citation

Prusa, Jerome; Missak, Johanna; Kittrell, Jeff; Evans, John J.; and Tapprich, William, "Major alteration in coxsackievirus B3 genomic RNA structure distinguishes a virulent strain from an a virulent strain" (2014). Biology Faculty Publications. 165.
https://digitalcommons.unomaha.edu/biofacpub/165