Author ORCID Identifier

Denton - https://orcid.org/0000-0003-2458-8147

Dall - https://orcid.org/0000-0002-7024-2502

Document Type

Article

Publication Date

2-24-2022

Publication Title

Lupus: An International Journal

Volume

31

Issue

4

First Page

415

Last Page

423

Abstract

Aim

Current treatment of Systemic Lupus Erythematosus (SLE) is suboptimal and causes broad immunosuppression. Therapeutic use of helminths or helminth products has been suggested for autoimmune diseases such as SLE. In the present study, we evaluated possible immunomodulating effects of adult body fluid (ABF) from Ascaris suum on peripheral blood mononuclear cells (PBMCs) from SLE patients in an ex vivo setup.

Methods

PBMCs from SLE patients and healthy controls (HC) were isolated and stimulated ex vivo with ABF and Toll-like receptor agonists or activators of the stimulator of interferon genes (STING) or mitochondrial antiviral signaling protein (MAVS) pathways. After 24 h of incubation, the cytokine profile was analyzed using ELISA and Meso Scale Discovery techniques.

Results

ABF suppressed production of IL-6, TNF-α, CXCL10, and IL-10 by PBMCs from SLE patients and HCs following stimulation with specific agonists. ABF also reduced IFN-у production by stimulated PBMCs from HCs.

Conclusions

Our data show that ABF has an immunomodulatory effect on the production of key cytokines in the pathogenesis of SLE. These results suggest that ABF or ABF components hold potential as a novel treatment option for SLE.

Comments

This is an Accepted Manuscript of an article published by Sage in Lupus on February 24, 2022, available online: https://doi.org/10.1177/09612033221080548

Reuse restricted to noncommercial and no derivative uses.

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