Document Type
Article
Publication Date
11-2017
Publication Title
G3
Volume
7
First Page
3797
Last Page
3808
Abstract
The ability of the human pathogenic fungus Candida albicans to switch between yeast-like and filamentous forms of growth has long been linked to pathogenesis. Numerous environmental conditions, including growth at high temperatures, nutrient limitation, and exposure to serum, can trigger this morphological switch and are frequently used in in vitro models to identify genes with roles in filamentation. Previous work has suggested that differences exist between the various in vitro models both in the genetic requirements for filamentation and transcriptional responses to distinct filamentation-inducing media, but these differences had not been analyzed in detail. We compared 10 in vitro models for filamentation and found broad genetic and transcriptomic differences between model systems. The comparative analysis enabled the discovery of novel media-independent genetic requirements for filamentation as well as a core filamentation transcriptional profile. Our data also suggest that the physical environment drives distinct programs of filamentation in C. albicans, which has significant implications for filamentation in vivo.
Recommended Citation
Azadmanesh, Jahaun; Gowen, Austin M.; Creger, Paul E.; Shafer, Nicole D.; and Blankenship, Jill R., "Filamentation Involves Two Overlapping, but Distinct, Programs of Filamentation in the Pathogenic Fungus Candida albicans" (2017). Biology Faculty Publications. 89.
https://digitalcommons.unomaha.edu/biofacpub/89
Funded by the University of Nebraska at Omaha Open Access Fund
Comments
© 2017 Azadmanesh et al. doi: https://doi.org/10.1534/g3.117.300224. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/ licenses/by/4.0/). Supplemental material is available online at www.g3journal.org/lookup/suppl/ doi:10.1534/g3.117.300224/-/DC1.