Author ORCID Identifier
Dotov https://orcid.org/0000-0002-5543-360X
López-Casillas - https://orcid.org/0000-0002-3689-1824
Document Type
Article
Publication Date
5-24-2023
Abstract
Background
Betaglycan, also known as the TGFβ type III receptor (Tgfbr3), is a co-receptor that modulates TGFβ family signaling. Tgfbr3 is upregulated during C2C12 myoblast differentiation and expressed in mouse embryos myocytes.
Results
To investigate tgfbr3 transcriptional regulation during zebrafish embryonic myogenesis, we cloned a 3.2 kb promoter fragment that drives reporter transcription during C2C12 myoblasts differentiation and in the Tg(tgfbr3:mCherry) transgenic zebrafish. We detect tgfbr3 protein and mCherry expression in the adaxial cells concomitantly with the onset of their radial migration to become slow-twitch muscle fibers in the Tg(tgfbr3:mCherry). Remarkably, this expression displays a measurable antero-posterior somitic gradient expression.
Conclusions
tgfbr3 is transcriptionally regulated during somitic muscle development in zebrafish with an antero-posterior gradient expression that preferentially marks the adaxial cells and their descendants.
DOI
https://doi.org/10.1002/dvdy.602
Journal Title
Developmental Dynamics
Volume
252
Issue
9
First Page
1162
Last Page
1179
Recommended Citation
Ramírez-Vidal, Lizbeth; Molina-Villa, Tonatiuh; Mendoza, Valentín; Peralta-Álvarez, Carlos Alberto; Poot-Hernández, Augusto Cesar; Dotov, Dobromir; and López-Casillas, Fernado, "Betaglycan promoter activity is differentially regulated during myogenesis in zebrafish embryo somites" (2023). Journal Articles. 379.
https://digitalcommons.unomaha.edu/biomechanicsarticles/379
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial 4.0 License
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