Plaque Assay of EMS exposed Toxoplasma gondii
Advisor Information
Paul Davis
Location
UNO Criss Library, Room 249
Presentation Type
Oral Presentation
Start Date
6-3-2015 1:45 PM
End Date
6-3-2015 2:00 PM
Abstract
Toxoplasma gondii is a parasite which resides in mammals. Toxoplasmosis has been shown to alter mouse behavior and is suspected to do so in humans. It has been shown to cause slower reaction times and reckless behavior. Finding a drug to eradicate Toxoplasma gondii could help decrease the effects of these behavior changes. Our group has identified potential new therapies for T. gondii, but the target of this drug is currently unknown. We will use mutagenesis and screening to identify these targets and this work describes the development of methods for generating mutagenized pathogen. To test the efficacy of our mutagenesis, we have used plaque assays. These plaque assays will help us determine whether or not we are successfully mutating T. gondii at varying mutagen doses.
Plaque Assay of EMS exposed Toxoplasma gondii
UNO Criss Library, Room 249
Toxoplasma gondii is a parasite which resides in mammals. Toxoplasmosis has been shown to alter mouse behavior and is suspected to do so in humans. It has been shown to cause slower reaction times and reckless behavior. Finding a drug to eradicate Toxoplasma gondii could help decrease the effects of these behavior changes. Our group has identified potential new therapies for T. gondii, but the target of this drug is currently unknown. We will use mutagenesis and screening to identify these targets and this work describes the development of methods for generating mutagenized pathogen. To test the efficacy of our mutagenesis, we have used plaque assays. These plaque assays will help us determine whether or not we are successfully mutating T. gondii at varying mutagen doses.