Presentation Title

Plaque Assay of EMS exposed Toxoplasma gondii

Advisor Information

Paul Davis

Location

UNO Criss Library, Room 249

Presentation Type

Oral Presentation

Start Date

6-3-2015 1:45 PM

End Date

6-3-2015 2:00 PM

Abstract

Toxoplasma gondii is a parasite which resides in mammals. Toxoplasmosis has been shown to alter mouse behavior and is suspected to do so in humans. It has been shown to cause slower reaction times and reckless behavior. Finding a drug to eradicate Toxoplasma gondii could help decrease the effects of these behavior changes. Our group has identified potential new therapies for T. gondii, but the target of this drug is currently unknown. We will use mutagenesis and screening to identify these targets and this work describes the development of methods for generating mutagenized pathogen. To test the efficacy of our mutagenesis, we have used plaque assays. These plaque assays will help us determine whether or not we are successfully mutating T. gondii at varying mutagen doses.

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Mar 6th, 1:45 PM Mar 6th, 2:00 PM

Plaque Assay of EMS exposed Toxoplasma gondii

UNO Criss Library, Room 249

Toxoplasma gondii is a parasite which resides in mammals. Toxoplasmosis has been shown to alter mouse behavior and is suspected to do so in humans. It has been shown to cause slower reaction times and reckless behavior. Finding a drug to eradicate Toxoplasma gondii could help decrease the effects of these behavior changes. Our group has identified potential new therapies for T. gondii, but the target of this drug is currently unknown. We will use mutagenesis and screening to identify these targets and this work describes the development of methods for generating mutagenized pathogen. To test the efficacy of our mutagenesis, we have used plaque assays. These plaque assays will help us determine whether or not we are successfully mutating T. gondii at varying mutagen doses.