Chemical Probe of a Critical Cytosine Cluster in the RNA Genome of Coxsackievirus
Advisor Information
William Tapprich
Location
Dr. C.C. and Mabel L. Criss Library
Presentation Type
Poster
Start Date
2-3-2018 9:00 AM
End Date
2-3-2018 10:15 AM
Abstract
Coxsackievirus B3 (CVB3) a picornavirus, is a causative pathogen for several human diseases including myocarditis and Type I diabetes .Previous studies with Polio Virus have identified a cytosine rich region in the 5’UTR (untranslated) RNA genome of Polio virus at positions 93-100 to be of importance to viral-host protein interactions necessary for viral proliferation. This particular region is highly conserved among enteroviruses such as CVB3 and Polio virus. In order to explore the role of the spacer region in CVB3/28 RNA genome we mutated two cytosine’s at positions 93-94 in the 5’UTR with adenosines. We then used chemical probing using Selective 2’- Hydroxyl Acylation analyzed by Primer Extension (SHAPE) to characterize the mutant CVB3/28 strain. Data from SHAPE reactivity values was used to generate 5’UTR structures.
Chemical Probe of a Critical Cytosine Cluster in the RNA Genome of Coxsackievirus
Dr. C.C. and Mabel L. Criss Library
Coxsackievirus B3 (CVB3) a picornavirus, is a causative pathogen for several human diseases including myocarditis and Type I diabetes .Previous studies with Polio Virus have identified a cytosine rich region in the 5’UTR (untranslated) RNA genome of Polio virus at positions 93-100 to be of importance to viral-host protein interactions necessary for viral proliferation. This particular region is highly conserved among enteroviruses such as CVB3 and Polio virus. In order to explore the role of the spacer region in CVB3/28 RNA genome we mutated two cytosine’s at positions 93-94 in the 5’UTR with adenosines. We then used chemical probing using Selective 2’- Hydroxyl Acylation analyzed by Primer Extension (SHAPE) to characterize the mutant CVB3/28 strain. Data from SHAPE reactivity values was used to generate 5’UTR structures.