Advisor Information
Dr. Dustin Slivka
Presentation Type
Poster
Start Date
1-3-2019 10:45 AM
End Date
1-3-2019 12:00 PM
Abstract
BACKGROUND: Different modes of exercise and temperatures can elicit different responses in mitochondrial gene expression. Stresses including exercise and cold exposure act to trigger the transcription of genes associated with mitochondrial growth (biogenesis). Traditional endurance exercise is known to have a potent effect on mitochondrial growth biogenesis. However, limited knowledge exists on the effect of local cold application during endurance exercise. Additionally, it is currently unknown how cold exposure impacts mitochondrial breakdown (mitophagy). PURPOSE: Therefore, the purpose of this study is to determine the impact of local muscle cooling during endurance exercise on human skeletal muscle gene expression related to mitochondrial homeostasis (mitophagy and biogenesis). METHODS: Twelve recreationally-trained males and females (age 19-45) will be recruited for the study. Subjects will complete a 60-min cycling protocol at 65% Wpeak with thermal pads applied to their legs followed by 4 h of recovery. One leg will be cooled (ICE) and the other leg will be kept at thermoneutral conditions (CON). The leg that is cooled and the leg that serves as the control will be randomized. Intramuscular temperature will be recorded continuously. Intramuscular temperature will be recorded continuously. Muscle biopsies will be taken from each vastus lateralis before and after the cycling as well as at 4 hours post-exercise for the analysis of mitochondrial-related gene expression. IMPLICATIONS: This data may establish the effectiveness of using local cooling during endurance exercise. Furthermore, future research could determine the effectiveness of local cold application as a potential therapy for mitochondrial dysfunction.
Effect of Local Cold Application during Exercise on Mitochondrial Gene Expression
BACKGROUND: Different modes of exercise and temperatures can elicit different responses in mitochondrial gene expression. Stresses including exercise and cold exposure act to trigger the transcription of genes associated with mitochondrial growth (biogenesis). Traditional endurance exercise is known to have a potent effect on mitochondrial growth biogenesis. However, limited knowledge exists on the effect of local cold application during endurance exercise. Additionally, it is currently unknown how cold exposure impacts mitochondrial breakdown (mitophagy). PURPOSE: Therefore, the purpose of this study is to determine the impact of local muscle cooling during endurance exercise on human skeletal muscle gene expression related to mitochondrial homeostasis (mitophagy and biogenesis). METHODS: Twelve recreationally-trained males and females (age 19-45) will be recruited for the study. Subjects will complete a 60-min cycling protocol at 65% Wpeak with thermal pads applied to their legs followed by 4 h of recovery. One leg will be cooled (ICE) and the other leg will be kept at thermoneutral conditions (CON). The leg that is cooled and the leg that serves as the control will be randomized. Intramuscular temperature will be recorded continuously. Intramuscular temperature will be recorded continuously. Muscle biopsies will be taken from each vastus lateralis before and after the cycling as well as at 4 hours post-exercise for the analysis of mitochondrial-related gene expression. IMPLICATIONS: This data may establish the effectiveness of using local cooling during endurance exercise. Furthermore, future research could determine the effectiveness of local cold application as a potential therapy for mitochondrial dysfunction.