Presenter Information

Halee KellerFollow

Advisor Information

Dustin Slivka

Presentation Type

Poster

Start Date

1-3-2019 10:45 AM

End Date

1-3-2019 12:00 PM

Abstract

Halee Keller, Robert Shute, Dustin Slivka

University of Nebraska at Omaha, School of Health and Kinesiology, Omaha, NE

BACKGROUND: Mitochondria are highly concentrated in skeletal muscle tissue, and undergo damage from ROS during metabolic processes. This damage is often left unrepaired and leads to mitochondrial dysfunction, which has been linked to many common diseases. Exercise training increases mitochondrial development within skeletal muscle tissue and thus may be protective. Environmental temperature, when paired with exercise may provide an even greater effect than exercise alone. PURPOSE: The purpose of this study is to analyze the effects of three weeks of exercise training with various environmental temperature conditions on mitochondrial quantity and quality. METHODS: Thirty-six male subjects performed one hour of cycling five days/week for three weeks in either a hot (33° C), cold (7° C), or neutral (20° C) condition. Biopsies were taken from the vastus lateralis muscle for analysis of mitochondrial quantity and quality on the first and last day of training (pre, post, and 4-hrs post exercise). Thus, we can calculate mitochondrial copy number and deletion ratio. RESULTS: mtMinArc and mtMajArc copy number did not change within any temperature condition before or after 3 weeks training (p>0.05). mtDNA deletion ratio was lower in the cold compared to both hot (pCONCLUSIONS:These data indicate that the adaptive effects of exercise in three weeks do not cause a change in mitochondrial quantity. However, in cold conditions the lower mtDNA deletion ratio implies mitochondrial remodeling, which results in higher mitochondrial quality.

COinS
 
Mar 1st, 10:45 AM Mar 1st, 12:00 PM

IMPACTS OF EXERCISE AND ENVIRONMENTAL TEMPERATURE ON MITOCHONDRIAL QUANTITY AND QUALITY

Halee Keller, Robert Shute, Dustin Slivka

University of Nebraska at Omaha, School of Health and Kinesiology, Omaha, NE

BACKGROUND: Mitochondria are highly concentrated in skeletal muscle tissue, and undergo damage from ROS during metabolic processes. This damage is often left unrepaired and leads to mitochondrial dysfunction, which has been linked to many common diseases. Exercise training increases mitochondrial development within skeletal muscle tissue and thus may be protective. Environmental temperature, when paired with exercise may provide an even greater effect than exercise alone. PURPOSE: The purpose of this study is to analyze the effects of three weeks of exercise training with various environmental temperature conditions on mitochondrial quantity and quality. METHODS: Thirty-six male subjects performed one hour of cycling five days/week for three weeks in either a hot (33° C), cold (7° C), or neutral (20° C) condition. Biopsies were taken from the vastus lateralis muscle for analysis of mitochondrial quantity and quality on the first and last day of training (pre, post, and 4-hrs post exercise). Thus, we can calculate mitochondrial copy number and deletion ratio. RESULTS: mtMinArc and mtMajArc copy number did not change within any temperature condition before or after 3 weeks training (p>0.05). mtDNA deletion ratio was lower in the cold compared to both hot (pCONCLUSIONS:These data indicate that the adaptive effects of exercise in three weeks do not cause a change in mitochondrial quantity. However, in cold conditions the lower mtDNA deletion ratio implies mitochondrial remodeling, which results in higher mitochondrial quality.