Advisor Information
Dr. Dustin Slivka
Location
Criss Library
Presentation Type
Poster
Start Date
1-3-2019 9:00 AM
End Date
1-3-2019 10:15 AM
Abstract
BACKGROUND: The signaling pathways generated during exercise elicit mitochondrial adaptation, which includes both biogenesis and removal of damaged mitochondria (also known as mitophagy). It has been established that training followed by cold exposure alters the transcription of genes associated with mitochondrial growth and division. However, it is currently unknown whether temperature intervention during exercise influences the mRNA expression of genes associated with mitophagy, which is essential to maintaining the quality of this organelle. PURPOSE: To determine the expression of PINK1, PARK2, BNIP3, and BNIP3L mRNA following exercise in a cold environmental temperature with recovery at room temperature compared to exercise and recovery at room temperature. METHODS: Eleven recreationally trained males cycled at 65% Wpeak for an hour at -2 °C (cold) and 20 °C (room temperature). A muscle biopsy was taken from the vastus lateralis before exercise as well as 3h and 6h- post-exercise for gene expression analysis. RESULTS: Average exercise heart rate and skin temperature were lower in the cold compared to room temperature (154 ± 4 bpm, 160 ± 4 bpm, P = 0.004; 28.0 ± 0.4 °C, 33.7 ± 0.3 °C, P < 0.001, respectively), while core temperature was higher in the cold (38.9 ± 0.3 °C, 38.4 ± 0.2 °C, P = 0.016). BNIP3 and BNIP3L mRNA were not influenced by exercise or temperature (P > 0.05). PINK1 and PARK2 decreased 3h-post-exercise compared to pre-exercise (P = 0.002, P = 0.001, respectively). This response was diminished 6h-post-exercise, but values were still below baseline (P < 0.05). CONCLUSION: Gene expression for select genes associated with mitophagy (PINK1, PARK2) is decreased after exercise with no difference between cycling in the cold compared to room temperature.
Skeletal Muscle Mitophagy In Response To Cold Exposure During Exercise
Criss Library
BACKGROUND: The signaling pathways generated during exercise elicit mitochondrial adaptation, which includes both biogenesis and removal of damaged mitochondria (also known as mitophagy). It has been established that training followed by cold exposure alters the transcription of genes associated with mitochondrial growth and division. However, it is currently unknown whether temperature intervention during exercise influences the mRNA expression of genes associated with mitophagy, which is essential to maintaining the quality of this organelle. PURPOSE: To determine the expression of PINK1, PARK2, BNIP3, and BNIP3L mRNA following exercise in a cold environmental temperature with recovery at room temperature compared to exercise and recovery at room temperature. METHODS: Eleven recreationally trained males cycled at 65% Wpeak for an hour at -2 °C (cold) and 20 °C (room temperature). A muscle biopsy was taken from the vastus lateralis before exercise as well as 3h and 6h- post-exercise for gene expression analysis. RESULTS: Average exercise heart rate and skin temperature were lower in the cold compared to room temperature (154 ± 4 bpm, 160 ± 4 bpm, P = 0.004; 28.0 ± 0.4 °C, 33.7 ± 0.3 °C, P < 0.001, respectively), while core temperature was higher in the cold (38.9 ± 0.3 °C, 38.4 ± 0.2 °C, P = 0.016). BNIP3 and BNIP3L mRNA were not influenced by exercise or temperature (P > 0.05). PINK1 and PARK2 decreased 3h-post-exercise compared to pre-exercise (P = 0.002, P = 0.001, respectively). This response was diminished 6h-post-exercise, but values were still below baseline (P < 0.05). CONCLUSION: Gene expression for select genes associated with mitophagy (PINK1, PARK2) is decreased after exercise with no difference between cycling in the cold compared to room temperature.