Advisor Information
Dustin Slivka
Location
Criss Library
Presentation Type
Poster
Start Date
1-3-2019 12:30 PM
End Date
1-3-2019 1:45 PM
Abstract
BACKGROUND: Aerobic exercise triggers mitochondrial biogenesis, the generation of new mitochondria, by ATP depletion and subsequent activation of the AMPK signaling pathway. This leads to enhanced expression of PGC-1α, a positive regulator of respiration, mitochondrial biogenesis, and adaptive thermogenesis. Previous research from our lab has shown that exercise followed by cold recovery enhances the transcription of genes associated with mitochondrial growth and division, however the recovery period was necessary for this response. Perhaps exercising in a colder external environment would induce a greater thermoregulatory response and gradient between ambient skin and core temperature that may lead to altered mitochondrial turnover. These data may help exercise-induced mitochondrial biogenesis response and thus aid in the development of temperature-optimized training protocols to combat mitochondrial dysfunction. PURPOSE: To examine the mRNA expression of PGC-1α and other select genes related to mitochondrial biogenesis after exercise in a cold environmental temperature compared to exercise in room temperature. METHODS: Eleven recreationally trained males cycled at 65% Wpeak for an hour at -2°C and 20°C in a random, counterbalanced order. A muscle biopsy was taken from the vastus lateralis pre-exercise as well as 3-h and 6-h post-exercise for analysis of genes associated with mitochondrial biogenesis (PPGC-1α, GABPA, ERRα, NRF1, TFAM, and VEGF). RESULTS: VEGF and PGC-1α increased with exercise and remained elevated compared to pre-exercise a both post-exercise time points (P<0.05). NRF1 was lower after cycling regardless of temperature (P = 0.005, P<0.001, respectively). GABPA and ERRα decreased 3h-post-exercise (P=0.032. P=0.003, respectively) and were elevated at 6h-post exercise compared to 3h-post-exercise (P=0.017, P=0.005, respectively). TFAM expression was increased at 6h-post-exercise compared to both pre-exercise and 3-h-post-exercise levels (P=0.021, P<0.001, respectively). CONCLUSION: Gene expression related to mitochondrial biogenesis is altered after exercise with no difference between trials at -2°C and 20°C.
SKELETAL MUSCLE MITOCHONDRIAL BIOGENESIS IN RESPONSE TO EXERCISE AND COLD EXPOSURE
Criss Library
BACKGROUND: Aerobic exercise triggers mitochondrial biogenesis, the generation of new mitochondria, by ATP depletion and subsequent activation of the AMPK signaling pathway. This leads to enhanced expression of PGC-1α, a positive regulator of respiration, mitochondrial biogenesis, and adaptive thermogenesis. Previous research from our lab has shown that exercise followed by cold recovery enhances the transcription of genes associated with mitochondrial growth and division, however the recovery period was necessary for this response. Perhaps exercising in a colder external environment would induce a greater thermoregulatory response and gradient between ambient skin and core temperature that may lead to altered mitochondrial turnover. These data may help exercise-induced mitochondrial biogenesis response and thus aid in the development of temperature-optimized training protocols to combat mitochondrial dysfunction. PURPOSE: To examine the mRNA expression of PGC-1α and other select genes related to mitochondrial biogenesis after exercise in a cold environmental temperature compared to exercise in room temperature. METHODS: Eleven recreationally trained males cycled at 65% Wpeak for an hour at -2°C and 20°C in a random, counterbalanced order. A muscle biopsy was taken from the vastus lateralis pre-exercise as well as 3-h and 6-h post-exercise for analysis of genes associated with mitochondrial biogenesis (PPGC-1α, GABPA, ERRα, NRF1, TFAM, and VEGF). RESULTS: VEGF and PGC-1α increased with exercise and remained elevated compared to pre-exercise a both post-exercise time points (P<0.05). NRF1 was lower after cycling regardless of temperature (P = 0.005, P<0.001, respectively). GABPA and ERRα decreased 3h-post-exercise (P=0.032. P=0.003, respectively) and were elevated at 6h-post exercise compared to 3h-post-exercise (P=0.017, P=0.005, respectively). TFAM expression was increased at 6h-post-exercise compared to both pre-exercise and 3-h-post-exercise levels (P=0.021, P<0.001, respectively). CONCLUSION: Gene expression related to mitochondrial biogenesis is altered after exercise with no difference between trials at -2°C and 20°C.