Effect of Local Cold Application during Exercise on Gene Expression Related to Mitochondrial Development
Advisor Information
Dr. Dustin Slivka
Location
MBSC 201
Presentation Type
Poster
Start Date
6-3-2020 10:45 AM
End Date
6-3-2020 12:00 PM
Abstract
BACKGROUND: A well-known adaptation to endurance exercise training is an increase in mitochondrial content in active skeletal muscle fibers. Previous work from our lab suggest that genes related to the mitochondria have a more favorable response to exercise in cold environments. However, it is unknown whether this is related to muscle temperature. PURPOSE: Therefore, the purpose of this study is to determine the impact of decreasing muscle temperature during endurance exercise on human skeletal muscle gene expression related to the mitochondria. METHODS: Twelve recreationally trained males and females (age 19-45) cycled at 65% Wpeak with thermal pads applied to their legs followed by 4 h of recovery. One leg was randomly chosen to be the cooled limb (C) by the thermal pad and the other leg had the thermal pad on but the pad was not cooled (N). Muscle biopsies was taken from each vastus lateralis before and 4 hours post-exercise for the analysis of mitochondrial-related gene expression. RESULTS: Muscle temperature was lower in C than N after pre-cooling for 30 minutes before exercise (29.2±0.7, 34.1±0.3, P < 0.05) and this difference remained after exercise (36.9±0.2, 38.4±0.1, P < 0.05). The temperature returned to baseline after recovery (34.5±0.2, 34.4±0.2, P < 0.05). There was an exercise effect in both legs with PGC-1α mRNA expression inhibited in C compared to N (P < 0.05). CONCLUSION: These data suggest that decreased muscle temperature has an inhibitory effect on exercise-induced gene expression in human skeletal muscle.
Effect of Local Cold Application during Exercise on Gene Expression Related to Mitochondrial Development
MBSC 201
BACKGROUND: A well-known adaptation to endurance exercise training is an increase in mitochondrial content in active skeletal muscle fibers. Previous work from our lab suggest that genes related to the mitochondria have a more favorable response to exercise in cold environments. However, it is unknown whether this is related to muscle temperature. PURPOSE: Therefore, the purpose of this study is to determine the impact of decreasing muscle temperature during endurance exercise on human skeletal muscle gene expression related to the mitochondria. METHODS: Twelve recreationally trained males and females (age 19-45) cycled at 65% Wpeak with thermal pads applied to their legs followed by 4 h of recovery. One leg was randomly chosen to be the cooled limb (C) by the thermal pad and the other leg had the thermal pad on but the pad was not cooled (N). Muscle biopsies was taken from each vastus lateralis before and 4 hours post-exercise for the analysis of mitochondrial-related gene expression. RESULTS: Muscle temperature was lower in C than N after pre-cooling for 30 minutes before exercise (29.2±0.7, 34.1±0.3, P < 0.05) and this difference remained after exercise (36.9±0.2, 38.4±0.1, P < 0.05). The temperature returned to baseline after recovery (34.5±0.2, 34.4±0.2, P < 0.05). There was an exercise effect in both legs with PGC-1α mRNA expression inhibited in C compared to N (P < 0.05). CONCLUSION: These data suggest that decreased muscle temperature has an inhibitory effect on exercise-induced gene expression in human skeletal muscle.