The Complete Mitochondrial Genome of Aeorestes Cineresus
Advisor Information
Jeremy White and Sarah Gaughan
Location
MBSC 201
Presentation Type
Poster
Start Date
6-3-2020 2:00 PM
End Date
6-3-2020 3:15 PM
Abstract
Mitochondrial genomes have been successfully used to infer population structure, genetic diversity, species identity, and phylogenetic relationships. This study is the first to sequence the complete mitochondrial genome of the hoary bat (Aeorestes cinereus) and provides much-needed insight into the evolutionary history of this species. Mitochondrial DNA was extracted from skeletal muscle of a hoary bat using the Abcam Mitochondrial DNA Isolation Kit for Tissue and subsequently sequenced on an Illumina NextSeq at the University of Nebraska Medical Center. Sequences were assembled using Geneious and annotated using DOGMA. MEGA X was used to build a phylogenetic tree comparing the mitochondrial genome of A. cinereus with the mitochondrial genomes of eight other bat species. The tree indicates that A. cinereus is in a sister clade to the eastern red bat (Lasiurus borealis). Establishing more genetic resources for this species will help to predict its conservation needs and susceptibility to anthropogenic threats.
The Complete Mitochondrial Genome of Aeorestes Cineresus
MBSC 201
Mitochondrial genomes have been successfully used to infer population structure, genetic diversity, species identity, and phylogenetic relationships. This study is the first to sequence the complete mitochondrial genome of the hoary bat (Aeorestes cinereus) and provides much-needed insight into the evolutionary history of this species. Mitochondrial DNA was extracted from skeletal muscle of a hoary bat using the Abcam Mitochondrial DNA Isolation Kit for Tissue and subsequently sequenced on an Illumina NextSeq at the University of Nebraska Medical Center. Sequences were assembled using Geneious and annotated using DOGMA. MEGA X was used to build a phylogenetic tree comparing the mitochondrial genome of A. cinereus with the mitochondrial genomes of eight other bat species. The tree indicates that A. cinereus is in a sister clade to the eastern red bat (Lasiurus borealis). Establishing more genetic resources for this species will help to predict its conservation needs and susceptibility to anthropogenic threats.