The Mitochondrial Genome of Mexican Free-tailed Bats may offer new insights in the fight against white-nose syndrome
Advisor Information
Dr. Jeremy White, Sarah Gaughan
Location
MBSC 201
Presentation Type
Poster
Start Date
6-3-2020 10:45 AM
End Date
6-3-2020 12:00 PM
Abstract
The Mexican free-tailed bat (Tadarida brasiliensis) is an abundant species found in much of Central and South America, Mexico, and the southern United States. Some populations are migratory, and individuals are occasionally found in the central United States as far north as Wyoming, South Dakota, Nebraska, and Iowa. The primary goal of this study was to sequence the mitochondrial genome of T. brasiliensis to assist in future research of this species. We extracted mitochondrial DNA using the Abcam Mitochondrial DNA Isolation Kit from skeletal muscle tissue of an individual T. brasiliensis that was found in Nebraska. The extracted DNA was then sequenced on an Illumina NextSeq at the University of Nebraska Medical Center. Next we assembled the sequence using Geneious, and annotated it using DOGMA. We are currently still analyzing the DNA sequence and the evolutionary position of this species in the family Molossidae based on its mitochondrial genome. Our mitochondrial sequence may be useful in future research on the evolutionary history of this group of bats as well as conservation efforts.
The Mitochondrial Genome of Mexican Free-tailed Bats may offer new insights in the fight against white-nose syndrome
MBSC 201
The Mexican free-tailed bat (Tadarida brasiliensis) is an abundant species found in much of Central and South America, Mexico, and the southern United States. Some populations are migratory, and individuals are occasionally found in the central United States as far north as Wyoming, South Dakota, Nebraska, and Iowa. The primary goal of this study was to sequence the mitochondrial genome of T. brasiliensis to assist in future research of this species. We extracted mitochondrial DNA using the Abcam Mitochondrial DNA Isolation Kit from skeletal muscle tissue of an individual T. brasiliensis that was found in Nebraska. The extracted DNA was then sequenced on an Illumina NextSeq at the University of Nebraska Medical Center. Next we assembled the sequence using Geneious, and annotated it using DOGMA. We are currently still analyzing the DNA sequence and the evolutionary position of this species in the family Molossidae based on its mitochondrial genome. Our mitochondrial sequence may be useful in future research on the evolutionary history of this group of bats as well as conservation efforts.