Advisor Information
Andrew Miller
Presentation Type
Poster
Start Date
26-3-2021 12:00 AM
End Date
26-3-2021 12:00 AM
Abstract
Atrazine is a commonly used herbicide in agriculture that has been shown to cause adverse health-effects on biological organisms. Glacier Creek Preserve (GCP), a prairie preserve near Omaha, NE, contains restored prairie and agricultural land uses within a single watershed. Surface water samples were collected at various locations of GCP from May to July to quantify atrazine concentrations. Sample preparation included filtering with a 0.45 micron filter and solid-phase extraction (SPE) with a reversed-phase cartridge. The analyte was eluted off the cartridge with ethyl acetate and analyzed by gas chromatography-mass spectrometry (GC/MS). Quantitation was performed with an internal standard calibration curve that had a limit of detection (LOD) of approximately 0.2 ppb. A quality control standard was prepared and analyzed (N = 3) by this method resulting in an average recovery of 106%. Other QA/QC methods comprised a check standard and sample spikes. Spatial and temporal relationships of atrazine were determined, and the concentration of atrazine at GCP never exceeded Environmental Protection Agency drinking water limits.
Determination of atrazine in Glacier Creek using gas chromatography-mass spectrometry
Atrazine is a commonly used herbicide in agriculture that has been shown to cause adverse health-effects on biological organisms. Glacier Creek Preserve (GCP), a prairie preserve near Omaha, NE, contains restored prairie and agricultural land uses within a single watershed. Surface water samples were collected at various locations of GCP from May to July to quantify atrazine concentrations. Sample preparation included filtering with a 0.45 micron filter and solid-phase extraction (SPE) with a reversed-phase cartridge. The analyte was eluted off the cartridge with ethyl acetate and analyzed by gas chromatography-mass spectrometry (GC/MS). Quantitation was performed with an internal standard calibration curve that had a limit of detection (LOD) of approximately 0.2 ppb. A quality control standard was prepared and analyzed (N = 3) by this method resulting in an average recovery of 106%. Other QA/QC methods comprised a check standard and sample spikes. Spatial and temporal relationships of atrazine were determined, and the concentration of atrazine at GCP never exceeded Environmental Protection Agency drinking water limits.