The Use of a Resazurin Reduction Assay and a Luciferin ATP Assay to Measure Splenocyte Activity
Advisor Information
Paul H. Davis
Presentation Type
Poster
Start Date
26-3-2021 12:00 AM
End Date
26-3-2021 12:00 AM
Abstract
Schistosomiasis is a condition caused by a parasitic worm infection that predominantly affects third world countries. There has been a novel drug developed that has demonstrated efficacy against worm infection. Preliminary data from our lab suggests that granulocyte surface markers are upregulated alongside these cells’ ability to produce reactive species in murine splenocytes post treatment. Through a resazurin reduction assay and a Luciferin ATP assay, we examined the effects of this novel drug on granulocytes’ ability to produce reactive oxygen species using ATP and NADH.
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The Use of a Resazurin Reduction Assay and a Luciferin ATP Assay to Measure Splenocyte Activity
Schistosomiasis is a condition caused by a parasitic worm infection that predominantly affects third world countries. There has been a novel drug developed that has demonstrated efficacy against worm infection. Preliminary data from our lab suggests that granulocyte surface markers are upregulated alongside these cells’ ability to produce reactive species in murine splenocytes post treatment. Through a resazurin reduction assay and a Luciferin ATP assay, we examined the effects of this novel drug on granulocytes’ ability to produce reactive oxygen species using ATP and NADH.