Integrative function of microcirculation and skeletal muscle function in peripheral artery disease
Advisor Information
Song-Young Park
Location
MBSC Dodge Room 302A - G
Presentation Type
Oral Presentation
Start Date
4-3-2022 2:00 PM
End Date
4-3-2022 3:15 PM
Abstract
Peripheral artery disease (PAD) is an atherosclerotic disease that impairs lower-extremity circulatory function. Attenuated skeletal muscle mitochondrial function and oxygen utility capacity have been reported in the ischemic limbs; however, the underlying mechanisms are not well-understood. We investigated the impacts of chronic ischemia on skeletal muscle arteriole vasodilatory function and its contribution to skeletal muscle mitochondrial function and microvascular oxygen delivery and utilization capacity (TOI) in PAD. Skeletal muscle and arteriole samples from patients with PAD (n=18, 68.4±10.2 years) and age-matched controls (CON, n=11, 64.6±9.3 years) were harvested. Endothelial-dependent and endothelial-independent vasodilatory function was assessed by flow, acetylcholine (ACh), and sodium nitroprusside (SNP), and skeletal muscle mitochondrial function was measured by high-resolution respirometry. TOI was assessed by near-infrared spectroscopy in-vivo. Endothelial-dependent vasodilation was attenuated in PAD in response to ACh (10-3M, CON: 71.1±7%, PAD: 45.5±6%, PP-3M, CON: 101.5±4%, PAD: 91.6±5%, P=0.12). Complex I + II state 3 respiration was lower in PAD (CON: 26.1±2.1, PAD: 7.8±1.4 pmol∙s-1∙mg-1, P-1, Pr=0.6 and r=0.5, respectively, Pr=0.5 and r=0.6, respectively, P
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Integrative function of microcirculation and skeletal muscle function in peripheral artery disease
MBSC Dodge Room 302A - G
Peripheral artery disease (PAD) is an atherosclerotic disease that impairs lower-extremity circulatory function. Attenuated skeletal muscle mitochondrial function and oxygen utility capacity have been reported in the ischemic limbs; however, the underlying mechanisms are not well-understood. We investigated the impacts of chronic ischemia on skeletal muscle arteriole vasodilatory function and its contribution to skeletal muscle mitochondrial function and microvascular oxygen delivery and utilization capacity (TOI) in PAD. Skeletal muscle and arteriole samples from patients with PAD (n=18, 68.4±10.2 years) and age-matched controls (CON, n=11, 64.6±9.3 years) were harvested. Endothelial-dependent and endothelial-independent vasodilatory function was assessed by flow, acetylcholine (ACh), and sodium nitroprusside (SNP), and skeletal muscle mitochondrial function was measured by high-resolution respirometry. TOI was assessed by near-infrared spectroscopy in-vivo. Endothelial-dependent vasodilation was attenuated in PAD in response to ACh (10-3M, CON: 71.1±7%, PAD: 45.5±6%, PP-3M, CON: 101.5±4%, PAD: 91.6±5%, P=0.12). Complex I + II state 3 respiration was lower in PAD (CON: 26.1±2.1, PAD: 7.8±1.4 pmol∙s-1∙mg-1, P-1, Pr=0.6 and r=0.5, respectively, Pr=0.5 and r=0.6, respectively, P