Influence of Local Muscle Cooling on Mitochondrial Related Gene Expression at Rest

Advisor Information

Dustin Slivka

Location

MBSC Ballroom - Poster #407 - G

Presentation Type

Poster

Start Date

4-3-2022 2:00 PM

End Date

4-3-2022 3:15 PM

Abstract

Local cold application has been shown to decrease the temperature of the skin and muscle, causing vasoconstriction, and decreasing blood flow. Currently, the effects of local cold application independent of exercise on mitochondrial related gene expression is unknown. Purpose: To determine the impact of localized cooling to the skeletal muscle during rest. Methods: Thermal wraps were applied to the vastus lateralis (VL) of each limb of 12 participants. One limb received a cold application (randomized) (COLD), while the other did not (CON). Wraps were removed at the 4-h time point and measurements of skin temperature, blood flow, intramuscular temperatures, and muscle biopsies were collected. RT-qPCR was used to measure expression of genes associated with mitochondrial development. Differences (pResults: Skin and muscle temperatures were lower in COLD than CON, pGABPA expression increased in COLD relative to CON (p=0.006). All other mitochondrial related gene expression had no statistical difference (PGC-1α, p=0.928; PGC-1α-1A, p=0.463; PGC-1α-NT, p=0.507; NRF1, p=0.396; TFAM, p=0.354; VEGF, p=0.200; ESRRα, p=0.894; PINK1, p=0.354; PARK2, p=0.400; BNIP3, p=0.659; BNIP3 L, p=0.649). Conclusion: While GABPA was statistically different (p=0.006), the magnitude of the change was small (1.34-fold). Therefore, there appears to be limited impact of local muscle cooling on the transcriptional response related to mitochondrial development. Supported by NE-INBRE, NIGMS Funding (P20GM103427).

This document is currently not available here.

COinS
 
Mar 4th, 2:00 PM Mar 4th, 3:15 PM

Influence of Local Muscle Cooling on Mitochondrial Related Gene Expression at Rest

MBSC Ballroom - Poster #407 - G

Local cold application has been shown to decrease the temperature of the skin and muscle, causing vasoconstriction, and decreasing blood flow. Currently, the effects of local cold application independent of exercise on mitochondrial related gene expression is unknown. Purpose: To determine the impact of localized cooling to the skeletal muscle during rest. Methods: Thermal wraps were applied to the vastus lateralis (VL) of each limb of 12 participants. One limb received a cold application (randomized) (COLD), while the other did not (CON). Wraps were removed at the 4-h time point and measurements of skin temperature, blood flow, intramuscular temperatures, and muscle biopsies were collected. RT-qPCR was used to measure expression of genes associated with mitochondrial development. Differences (pResults: Skin and muscle temperatures were lower in COLD than CON, pGABPA expression increased in COLD relative to CON (p=0.006). All other mitochondrial related gene expression had no statistical difference (PGC-1α, p=0.928; PGC-1α-1A, p=0.463; PGC-1α-NT, p=0.507; NRF1, p=0.396; TFAM, p=0.354; VEGF, p=0.200; ESRRα, p=0.894; PINK1, p=0.354; PARK2, p=0.400; BNIP3, p=0.659; BNIP3 L, p=0.649). Conclusion: While GABPA was statistically different (p=0.006), the magnitude of the change was small (1.34-fold). Therefore, there appears to be limited impact of local muscle cooling on the transcriptional response related to mitochondrial development. Supported by NE-INBRE, NIGMS Funding (P20GM103427).