Chitosan nanofibrous wound dressing accelerates wound healing via reduction of CD68+/CD163+ ratio and increase of angiogenesis and collagen deposition
Presenter Type
UNO Graduate Student (Doctoral)
Major/Field of Study
Biomechanics
Advisor Information
Assistant professor
Location
CEC RM #230
Presentation Type
Oral Presentation
Start Date
22-3-2024 10:30 AM
End Date
22-3-2024 11:45 AM
Abstract
Chitosan nanofibrous wound dressing accelerates wound healing via reduction of CD68+/CD163+ ratio and increase of angiogenesis and collagen deposition
Mahboubeh Ghanbari1, Mark A. Carlson2, Carlos P. Jara2, Yury Salkovskiy1
1Department of Biomechanics, University of Nebraska at Omaha, Omaha, NE, USA
2Department of Surgery, University of Nebraska Medical Center, NE, USA
email: mghanbari@unomaha.edu
Background: Chitosan-based materials hold significant potential for wound healing treatments due to their antibacterial properties, compatibility with living tissues, biodegradability, and low cost. Despite extensive research into chitosan applications for wound healing, the cellular and molecular mechanisms through which chitosan facilitates wound repair are not fully understood. This research aims to evaluate these mechanisms in a comparative study with commercial dressings using an acute excisional wound model in rats. Methods: Sprague Dawley rats (n=39) were divided into three main groups based on the type of dressings used. Two treatment groups received PriMatrix Dermal Repair Scaffold and chitosan nanofibrous dressings as the primary dressing, with a 3M Tegaderm transparent film applied as the secondary dressing; the third (control) group was treated solely with the Tegaderm dressing. Histological changes and collagen content were assessed using hematoxylin and eosin (H&E) and Masson's trichrome (MTC) staining, respectively. The tumor necrosis factor-alpha (TNF-α) levels and inducible nitric oxide synthase (iNOS) were measured in the wound tissue. Additionally, immunohistochemical staining for CD31, CD68, CD163, and vimentin was conducted on day 21 post-wounding. Results: The rate of epithelialization was significantly higher in rats treated with chitosan nanofibrous dressings (87.5% compared to 42.04%, p = 0.03) than in those treated with PriMatrix Scaffold. While TNF-α protein levels substantially decreased in rats treated with both chitosan nanofibrous dressings and PriMatrix Scaffold compared to the control group, iNOS levels increased only in the rats treated with chitosan nanofibrous dressings. The CD68+/CD163+ ratio was lower in the chitosan-treated rats (0.28 ± 0.04 vs. 0.58 ± 0.09, p = 0.02) than in those treated with PriMatrix Scaffold. Furthermore, collagen deposition and angiogenesis were higher in the chitosan nanofibrous-treated rats compared to the control group. Conclusion: Chitosan nanofibrous dressing significantly enhances cutaneous wound healing and is associated with pronounced neovascularization, increased collagen content, and the modulation of iNOS and TNF-α levels at the wound site.
Chitosan nanofibrous wound dressing accelerates wound healing via reduction of CD68+/CD163+ ratio and increase of angiogenesis and collagen deposition
CEC RM #230
Chitosan nanofibrous wound dressing accelerates wound healing via reduction of CD68+/CD163+ ratio and increase of angiogenesis and collagen deposition
Mahboubeh Ghanbari1, Mark A. Carlson2, Carlos P. Jara2, Yury Salkovskiy1
1Department of Biomechanics, University of Nebraska at Omaha, Omaha, NE, USA
2Department of Surgery, University of Nebraska Medical Center, NE, USA
email: mghanbari@unomaha.edu
Background: Chitosan-based materials hold significant potential for wound healing treatments due to their antibacterial properties, compatibility with living tissues, biodegradability, and low cost. Despite extensive research into chitosan applications for wound healing, the cellular and molecular mechanisms through which chitosan facilitates wound repair are not fully understood. This research aims to evaluate these mechanisms in a comparative study with commercial dressings using an acute excisional wound model in rats. Methods: Sprague Dawley rats (n=39) were divided into three main groups based on the type of dressings used. Two treatment groups received PriMatrix Dermal Repair Scaffold and chitosan nanofibrous dressings as the primary dressing, with a 3M Tegaderm transparent film applied as the secondary dressing; the third (control) group was treated solely with the Tegaderm dressing. Histological changes and collagen content were assessed using hematoxylin and eosin (H&E) and Masson's trichrome (MTC) staining, respectively. The tumor necrosis factor-alpha (TNF-α) levels and inducible nitric oxide synthase (iNOS) were measured in the wound tissue. Additionally, immunohistochemical staining for CD31, CD68, CD163, and vimentin was conducted on day 21 post-wounding. Results: The rate of epithelialization was significantly higher in rats treated with chitosan nanofibrous dressings (87.5% compared to 42.04%, p = 0.03) than in those treated with PriMatrix Scaffold. While TNF-α protein levels substantially decreased in rats treated with both chitosan nanofibrous dressings and PriMatrix Scaffold compared to the control group, iNOS levels increased only in the rats treated with chitosan nanofibrous dressings. The CD68+/CD163+ ratio was lower in the chitosan-treated rats (0.28 ± 0.04 vs. 0.58 ± 0.09, p = 0.02) than in those treated with PriMatrix Scaffold. Furthermore, collagen deposition and angiogenesis were higher in the chitosan nanofibrous-treated rats compared to the control group. Conclusion: Chitosan nanofibrous dressing significantly enhances cutaneous wound healing and is associated with pronounced neovascularization, increased collagen content, and the modulation of iNOS and TNF-α levels at the wound site.