Hydrogen Sulfide as a Biomarker in Patients with Peripheral Artery Disease

Presenter Type

UNO Graduate Student (Masters)

Major/Field of Study

Health and Kinesiology

Other

Health and Kinesiology with a focus in Exercise Physiology

Advisor Information

Associate Professor in Health and Physiology

Location

CEC RM #231

Presentation Type

Oral Presentation

Start Date

22-3-2024 2:30 PM

End Date

22-3-2024 3:45 PM

Abstract

H2S is a gaseous signaling molecule produced endogenously for a variety of functions. H2S plays roles in angiogenesis, vascular smooth muscle relaxation, endothelial function preservation, and redox balance, therefore, H2S is essential for maintaining cardiovascular health, reducing inflammation, and ensuring cellular homeostasis in aging. Several studies have noted that, with the aging process, comes a correlating decline in endogenous H2S levels in many different species including both mice and humans. However, aging mediated changes of H2S in vascular function have not been explored. Purpose: This study aims to examine the role of H2S in vascular and metabolic functions, as well as physical performance across different age groups, focusing on its implications for cardiovascular health in aging populations. Methods: The study involved 24 participants, categorized into three groups: healthy-young adults (YA, n = 10), healthy middle-aged adults (MA, n = 7), and healthy-older adults (OA, n = 7). Assessments included endothelial function with the flow-mediated dilation (FMD), arterial stiffness with pulse-wave velocity (PWV), skeletal muscle oxygen delivery and utilization capacity, the cardio-metabolic responses to graded exercise test, and peripheral blood mononuclear cell (PBMC) mitochondrial function. Results: Significant differences were observed in brachial FMD between YA and OA (p < 0.05), and in popliteal artery FMD between YA and MA (p < 0.05), and MA and OA (p < 0.05). Carotid-to-ankle PWV and carotid-to-femoral PWV also showed significant differences between YA and OA (p < 0.05), and between MA and OA (p < 0.05). Skeletal muscle oxygenation and utilization were significantly different between YA and OA (p < 0.05). However, no significant differences were found in PBMC mitochondrial respiration across age groups (p > 0.05). Conclusion: Significant age-related differences were found in endothelial function and arterial stiffness, as well as in muscle reoxygenation and metabolic rates, highlighting the impact of aging on vascular and muscle physiology. However, no significant differences in mitochondrial function across age groups were observed, suggesting either resilience to aging or the need for larger studies.

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COinS
 
Mar 22nd, 2:30 PM Mar 22nd, 3:45 PM

Hydrogen Sulfide as a Biomarker in Patients with Peripheral Artery Disease

CEC RM #231

H2S is a gaseous signaling molecule produced endogenously for a variety of functions. H2S plays roles in angiogenesis, vascular smooth muscle relaxation, endothelial function preservation, and redox balance, therefore, H2S is essential for maintaining cardiovascular health, reducing inflammation, and ensuring cellular homeostasis in aging. Several studies have noted that, with the aging process, comes a correlating decline in endogenous H2S levels in many different species including both mice and humans. However, aging mediated changes of H2S in vascular function have not been explored. Purpose: This study aims to examine the role of H2S in vascular and metabolic functions, as well as physical performance across different age groups, focusing on its implications for cardiovascular health in aging populations. Methods: The study involved 24 participants, categorized into three groups: healthy-young adults (YA, n = 10), healthy middle-aged adults (MA, n = 7), and healthy-older adults (OA, n = 7). Assessments included endothelial function with the flow-mediated dilation (FMD), arterial stiffness with pulse-wave velocity (PWV), skeletal muscle oxygen delivery and utilization capacity, the cardio-metabolic responses to graded exercise test, and peripheral blood mononuclear cell (PBMC) mitochondrial function. Results: Significant differences were observed in brachial FMD between YA and OA (p < 0.05), and in popliteal artery FMD between YA and MA (p < 0.05), and MA and OA (p < 0.05). Carotid-to-ankle PWV and carotid-to-femoral PWV also showed significant differences between YA and OA (p < 0.05), and between MA and OA (p < 0.05). Skeletal muscle oxygenation and utilization were significantly different between YA and OA (p < 0.05). However, no significant differences were found in PBMC mitochondrial respiration across age groups (p > 0.05). Conclusion: Significant age-related differences were found in endothelial function and arterial stiffness, as well as in muscle reoxygenation and metabolic rates, highlighting the impact of aging on vascular and muscle physiology. However, no significant differences in mitochondrial function across age groups were observed, suggesting either resilience to aging or the need for larger studies.