IMPROVING AN OPEN-SOURCED AUTOMATED MICROPLATE ASSAY FOR OUR DRUG DISCOVERY PROCESS
Presenter Type
UNO Undergraduate Student
Major/Field of Study
Chemistry
Other
Medicinal Chemistry
Advisor Information
Dr. Paul H. Davis pdavis@unomaha.edu
Location
CEC RM #116
Presentation Type
Oral Presentation
Start Date
22-3-2024 9:00 AM
End Date
22-3-2024 10:15 AM
Abstract
IMPROVING AN OPEN-SOURCED AUTOMATED MICROPLATE ASSAY FOR OUR DRUG DISCOVERY PROCESS
M. Yunos Alizai1, Brianna N. Davis1, and Paul H. Davis1 yalizai@unomaha.edu
1 - University of Nebraska at Omaha, Omaha, NE.
Microplate assays are important tools for anti-infective compound screening, cytotoxicity screening, and drug discovery. These microplate assays provide valuable insights into the efficacy of anti-infective compounds and their potential for use in the drug discovery process. These assays help determine the effectiveness of a substance or compound against pathogens and information on the potential toxicity of a substance or compound by measuring impacts on host cell metabolism, aiding in the evaluation of their suitability for clinical applications in combating infections. However, these assays take time and suffer from user-associated variations when testing multiple compounds for multiple pathogens and host cells. The development of high-throughput screening methods for compound assays is essential for the rapid evaluation of potential compounds in the drug discovery process. In this study, we describe the development process of an automated wide-spectrum screening assay for anti-infection compounds assay utilizing the OpenTrons robot. This assay can process a large number of samples in a reduced period of time and the automation reduces human error. To date, our findings support the feasibility of using the OpenTrons robot for an automated compound screening assay, providing a valuable tool for the drug discovery process.
IMPROVING AN OPEN-SOURCED AUTOMATED MICROPLATE ASSAY FOR OUR DRUG DISCOVERY PROCESS
CEC RM #116
IMPROVING AN OPEN-SOURCED AUTOMATED MICROPLATE ASSAY FOR OUR DRUG DISCOVERY PROCESS
M. Yunos Alizai1, Brianna N. Davis1, and Paul H. Davis1 yalizai@unomaha.edu
1 - University of Nebraska at Omaha, Omaha, NE.
Microplate assays are important tools for anti-infective compound screening, cytotoxicity screening, and drug discovery. These microplate assays provide valuable insights into the efficacy of anti-infective compounds and their potential for use in the drug discovery process. These assays help determine the effectiveness of a substance or compound against pathogens and information on the potential toxicity of a substance or compound by measuring impacts on host cell metabolism, aiding in the evaluation of their suitability for clinical applications in combating infections. However, these assays take time and suffer from user-associated variations when testing multiple compounds for multiple pathogens and host cells. The development of high-throughput screening methods for compound assays is essential for the rapid evaluation of potential compounds in the drug discovery process. In this study, we describe the development process of an automated wide-spectrum screening assay for anti-infection compounds assay utilizing the OpenTrons robot. This assay can process a large number of samples in a reduced period of time and the automation reduces human error. To date, our findings support the feasibility of using the OpenTrons robot for an automated compound screening assay, providing a valuable tool for the drug discovery process.